The subpopulation of CD44-positive cells promoted tumorigenicity and metastatic ability in lung adenocarcinoma
| Autor: | Chao-Yu Liu, Chi-Shuan Huang, Yi-Ping Yang, Yuh-Lih Chang, Wai-Wah Wu, Hsin-Chi Lin, Kai-Hsi Lu, Shou-Dong Lee, Yung-Yang Liu, Kuan-Hsuan Chen, Chien-Ying Wang |
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| Rok vydání: | 2019 |
| Předmět: |
Epithelial-Mesenchymal Transition
Lung Neoplasms Adenocarcinoma of Lung Snail Tumor initiation 030204 cardiovascular system & hematology 03 medical and health sciences 0302 clinical medicine Cell Line Tumor biology.animal medicine Humans Neoplasm Metastasis Lung cancer Lung Transition (genetics) biology business.industry CD44 Cancer General Medicine medicine.disease Hyaluronan Receptors medicine.anatomical_structure 030220 oncology & carcinogenesis Neoplastic Stem Cells Cancer research biology.protein Adenocarcinoma business |
| Zdroj: | Journal of the Chinese Medical Association. 82:196-201 |
| ISSN: | 1726-4901 |
| Popis: | BACKGROUND Lung cancer is one of the major causes of carcinoma-related deaths in the world. Importantly, lung adenocarcinoma (LAC) is the most common type with poor outcome. However, the progressive clinical phenotype and biomolecular signature of lung cancer presenting the cancer stem-like and metastatic characteristics are still unclear. METHODS In this study, we identified CD44 marker in lung cancers. The capabilities, including tumorigenic and migration assays, were analyzed in CD44 expression and CD44 expression subgroups. Meanwhile, the potential bio-signature and properties of lung tumor stem-like cells were further studied. RESULTS The high expression of CD44 subpopulation (CD44-positive) in isolated lung cancer cells showed significantly higher abilities of tumorigenic colonies, tumor-sphere formation, and migratory properties when compared with the CD44 expression group. These subgroups of CD44-positive lung cancer cells further demonstrated the metastatic potential with epithelial-mesenchymal transition (EMT), as well as the high expression of Twist and Snail gene profile. Importantly, the overexpression of Snail with gene vector in CD44 expression cells further significantly promoted the properties of lung tumor stem-like cells. CONCLUSION The results of this study highlighted the role of CD44-posivite subpopulation in modulating tumor initiation and EMT-based metastatic ability of lung malignancy. |
| Databáze: | OpenAIRE |
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