The influence of pH and ionic strength on the coating of peptides of herpes simplex virus type 1 in an enzyme-linked immunosorbent assay
| Autor: | H.J. Geerligs, Sytske Welling-Wester, W.J. Weijer, G.W. Welling, W. Bloemhoff |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1988 |
| Předmět: |
Immunology
Dose-Response Relationship Immunologic Peptide Enzyme-Linked Immunosorbent Assay Sodium Chloride medicine.disease_cause Antigen-Antibody Reactions chemistry.chemical_compound medicine Immunology and Allergy Simplexvirus Antigens Viral chemistry.chemical_classification biology Osmolar Concentration Antibody titer Hydrogen-Ion Concentration Herpes simplex virus Enzyme Biochemistry chemistry Ionic strength biology.protein Glutaraldehyde Antibody Glycoprotein Peptides |
| Zdroj: | Journal of Immunological Methods, 106(2), 239-244. ELSEVIER SCIENCE BV |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(88)90203-7 |
| Popis: | Rabbits were immunized with synthetic peptides of herpes simplex virus type 1 glycoproteins, coupled to a carrier protein with glutaraldehyde. Antibodies directed against the peptides were determined in an enzyme-linked immunosorbent assay (ELISA). Either free peptides or peptides coupled with glutaraldehyde to another carrier protein than the one used for immunization were used as the coating antigen. When conjugated peptides were used as the coat, it was necessary in some instances to correct the antibody titers for a substantial amount of antibody activity against glutaraldehyde. When free peptides were used, optimal coating conditions with regard to pH and ionic strength had to be determined, since some peptides failed to coat under standard conditions, at pH 9.6. The results show that some peptides needed stringent pH conditions while others could be coated in a broad pH range. The addition of 0.6 M NaCl had a favorable effect on peptide coating. |
| Databáze: | OpenAIRE |
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