Demonstration of apoptosis in neuroblastoma and its relationship to tumour regression
Autor: | Hirotaka Koizumi, Koonosuke Nakada, Munechika Wakisaka, Noboru Yamate, Toshifumi Takakuwa, Toshiyuki Uchikoshi, Teruhiro Fujioka |
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Rok vydání: | 1995 |
Předmět: |
Adolescent
Cell Apoptosis Biology Pathology and Forensic Medicine Neuroblastoma Proto-Oncogene Proteins medicine Humans fas Receptor Fragmentation (cell biology) Child Molecular Biology Infant Newborn Infant Cell Biology General Medicine DNA Neoplasm Fas receptor medicine.disease Molecular biology medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Neoplasm Regression Spontaneous Child Preschool DNA fragmentation Immunohistochemistry Immunostaining |
Zdroj: | Virchows Archiv : an international journal of pathology. 427(2) |
ISSN: | 0945-6317 |
Popis: | The in vivo occurrence of apoptosis in neuroblastomas was investigated. Histologically, a number of tumour cells showed typical apoptotic changes, including cell shrinkage, condensed and fragmented nuclei, eosinophilic cytoplasm, and absence of the inflammatory response. These cells coincided closely with the so-called karyorrhectic cells. An electrophoretic DNA ladder, a functional hallmark of apoptosis, was demonstrated in four of six tumours, and DNA fragmentation was detected in situ by terminal deoxytransferase-mediated nick end-labelling in 26 of 35 tumour specimens (74%). The labelled cell counts ranged from 5 to 62 per 5000 tumour cells (mean +/- SD: 15.0 +/- 14.5). Immunoperoxidase staining revealed that an apoptosis-suppressing protein, bcl-2, was expressed abundantly in advanced-stage tumours, whereas it was absent from karyorrhectic-apoptotic cells. Several tumours with the potential for spontaneous regression were bcl-2-deficient. Immunostaining of the Fas receptor for apoptosis demonstrated that the tumour cells expressed this molecule on their cell surfaces. Our results provide evidence of apoptosis in neuroblastomas and suggest that bcl-2 and the Fas receptor may play a role in its regulatory mechanisms. |
Databáze: | OpenAIRE |
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