Metabolomic profiling to characterize acute intestinal ischemia/reperfusion injury

Autor: Karen L. Madsen, Victor Tso, Saad Y. Salim, Thomas A. Churchill, Chris M. Lukowski, Rachel G. Khadaroo
Rok vydání: 2016
Předmět:
0301 basic medicine
Critical Care and Emergency Medicine
Physiology
Metabolite
lcsh:Medicine
Urine
Pathology and Laboratory Medicine
Gastroenterology
Biochemistry
Vascular Medicine
chemistry.chemical_compound
Mice
0302 clinical medicine
Mathematical and Statistical Techniques
Ischemia
Metabolites
Medicine and Health Sciences
lcsh:Science
Principal Component Analysis
Multidisciplinary
Discriminant Analysis
Body Fluids
Acute Intestinal Ischemia
Intestines
030220 oncology & carcinogenesis
Shock (circulatory)
Reperfusion Injury
Physical Sciences
medicine.symptom
Anatomy
Statistics (Mathematics)
Research Article
medicine.medical_specialty
Urinary system
Research and Analysis Methods
Sepsis
03 medical and health sciences
Signs and Symptoms
Diagnostic Medicine
Internal medicine
medicine
Animals
Metabolomics
Statistical Methods
Septic shock
business.industry
lcsh:R
Biology and Life Sciences
medicine.disease
Gastrointestinal Tract
030104 developmental biology
Metabolism
chemistry
Reperfusion
Multivariate Analysis
lcsh:Q
business
Reperfusion injury
Digestive System
Mathematics
Zdroj: PLoS ONE
PLoS ONE, Vol 12, Iss 6, p e0179326 (2017)
ISSN: 1932-6203
Popis: Sepsis and septic shock are the leading causes of death in critically ill patients. Acute intestinal ischemia/reperfusion (AII/R) is an adaptive response to shock. The high mortality rate from AII/R is due to the severity of the disease and, more importantly, the failure of timely diagnosis. The objective of this investigation is to use nuclear magnetic resonance (NMR) analysis to characterize urine metabolomic profile of AII/R injury in a mouse model. Animals were exposed to sham, early (30 min) or late (60 min) acute intestinal ischemia by complete occlusion of the superior mesenteric artery, followed by 2 hrs of reperfusion. Urine was collected and analyzed by NMR spectroscopy. Urinary metabolite concentrations demonstrated that different profiles could be delineated based on the duration of the intestinal ischemia. Metabolites such as allantoin, creatinine, proline, and methylamine could be predictive of AII/R injury. Lactate, currently used for clinical diagnosis, was found not to significantly contribute to the classification model for either early or late ischemia. This study demonstrates that patterns of changes in urinary metabolites are effective at distinguishing AII/R progression in an animal model. This is a proof-of-concept study to further support examination of metabolites in the clinical diagnosis of intestinal ischemia reperfusion injury in patients. The discovery of a fingerprint metabolite profile of AII/R will be a major advancement in the diagnosis, treatment, and prevention of systemic injury in critically ill patients.
Databáze: OpenAIRE
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