Rift Valley fever virus-induced encephalomyelitis and hepatitis in calves
| Autor: | M. J. Topper, J. C. Morrill, M. K. Rippy, C. A. Mebus |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Rift Valley Fever 040301 veterinary sciences Encephalomyelitis Central nervous system Cattle Diseases Viremia Spleen Biology Antibodies Viral Virus 0403 veterinary science Immunoenzyme Techniques 03 medical and health sciences medicine Animals Rift Valley fever Antigens Viral General Veterinary 04 agricultural and veterinary sciences medicine.disease Rift Valley fever virus Virology 030104 developmental biology medicine.anatomical_structure Cattle Meningitis Encephalitis |
| Zdroj: | Veterinary pathology. 29(6) |
| ISSN: | 0300-9858 |
| Popis: | Three calves (Nos. 1, 2 = 7 days old; No. 3 = 21 days old) were inoculated subcutaneously with virulent Rift Valley fever (RVF) virus. All calves became viremic and clinically ill, but the two 7-day-old calves were moribund and were euthanatized subsequently on post-inoculation day (PID) 3. Highest viral titers were measured in the serum, with lesser concentrations in the brain, heart, spleen, and liver of these animals. Viral antigens were detected by immunohistochemical analysis only in the livers, where positive staining was localized in coalescing foci of hepatocellular necrosis. The 21-day-old calf appeared to recover after viremia and pyrexia but became lethargic and ataxic and was euthanatized on PID 9. The calf was no longer viremic, and RVF virus was isolated only from the brain. Microscopic examination of the central nervous system revealed diffuse perivascular infiltrates of lymphocytes and macrophages, multifocal meningitis, and focal areas of neuronal necrosis and aggregates of macrophages, lymphocytes, and neutrophils throughout all regions of the brain and cervical spinal cord. There was positive immunohistochemical staining for viral antigens within the cytoplasm of neurons and glial cells throughout the central nervous system. Thus, RVF virus can cause encephalomyelitis in calves, and the specific virologic diagnosis can be made by immunohistochemical localization of viral antigens in formalin-fixed tissues. |
| Databáze: | OpenAIRE |
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