VEGF-A, VEGFR-1, VEGFR-2 and Tie2 levels in plasma of premature infants: relationship to retinopathy of prematurity
| Autor: | Christina Pieh, Marcus Krüger, Jürgen Schulte-Mönting, Wolf A. Lagrèze, Joachim Drevs, Hansjürgen Agostini, Ute Zirrgiebel, Christiane Buschbeck |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty Angiogenesis Gestational Age Gastroenterology Cellular and Molecular Neuroscience chemistry.chemical_compound Internal medicine medicine Humans Retinopathy of Prematurity Prospective Studies Analysis of Variance Vascular Endothelial Growth Factor Receptor-1 biology business.industry Infant Newborn Postmenstrual Age Retinal Retinopathy of prematurity Infant Low Birth Weight medicine.disease Receptor TIE-2 Vascular Endothelial Growth Factor Receptor-2 eye diseases Sensory Systems Vascular endothelial growth factor Ophthalmology Endocrinology chemistry Monoclonal Disease Progression biology.protein sense organs Analysis of variance Antibody business Infant Premature |
| Zdroj: | British Journal of Ophthalmology. 92:689-693 |
| ISSN: | 0007-1161 |
| DOI: | 10.1136/bjo.2007.128371 |
| Popis: | Aim: To study prospectively the plasma levels of vascular endothelial growth factor (VEGF-A), its soluble receptors sVEGFR-1, sVEGFR-2 and soluble Tie2 in premature infants. To identify their changes related to the onset of retinopathy of prematurity (ROP). Methods: Blood samples of 63 preterm infants born at a postmenstrual age (PMA) of 23–32 weeks were obtained between 5 days and 15 weeks after birth. 42 infants had no ROP, two had stage 1, nine stage 2 and 10 stage 3. Of these, four infants were treated with retinal photocoagulation. VEGF-A, sVEGFR-1, sVEGFR-2, and sTie2 were measured in the plasma with a sandwich enzyme immunoassay using factor-specific monoclonal mouse antibodies. The time course of concentrations plotted by kernel smoothing in infants with and without ROP were compared and a paired subgroup with analysis of variance was analysed. Results: ROP patients had raised plasma levels of sVEGFR-2 and sTie2 compared with premature infants without ROP. VEGF-A and sVEGFR-1 levels were similar in both groups. Analysis of a subgroup with pairs of measurements, one before 32 weeks and one after 36 weeks, showed a significant increase in sTie2 after 36 weeks of PMA independent of ROP (p = 0.03). Conclusion: This is the first study to measure plasma levels of angiogenic factors in ROP. Similar VEGF-A plasma levels in infants with and without ROP suggest that pathogenic retinal angiogenesis in ROP is mainly driven by local VEGF-A synthesis. Elevated plasma levels in active ROP were observed for sVEGFR-2 and sTie2. These increases have yet to be confirmed as predictive values for ROP. |
| Databáze: | OpenAIRE |
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