Oral administration of Hsp65-producing Lactococcus lactis attenuates allergic asthma in a murine model
| Autor: | Ana Paula Masson, M C R Barbosa, Thamires Milani, L B de Lacerda, Anderson Miyoshi, L N Z Ramalho, Marcos C. Borges, Wendy Martin Rios, Izaíra T. Brandão, Célio Lopes Silva |
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| Rok vydání: | 2020 |
| Předmět: |
Ovalbumin
medicine.medical_treatment Administration Oral Immunoglobulin E Applied Microbiology and Biotechnology T-Lymphocytes Regulatory Allergic inflammation Mice Immune system Bacterial Proteins Heat shock protein medicine Hypersensitivity Animals Pulmonary Eosinophilia Lung HIPERSENSIBILIDADE Inflammation Mice Inbred BALB C biology business.industry General Medicine Immunotherapy Chaperonin 60 respiratory system Asthma Lactococcus lactis Disease Models Animal Cytokine Immunoglobulin G Immunology biology.protein Cytokines Female business Bronchoalveolar Lavage Fluid Biotechnology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1365-2672 |
| Popis: | Aims Allergic asthma is a chronic inflammatory lung disease characterized by a Th2-type immune response pattern. The development of nonspecific immunotherapy is one of the primary goals for the control of this disease. Methods and results In this study, we evaluated the therapeutic effects of Lactococcus lactis-producing mycobacterial heat shock protein 65 (LLHsp65) in an ovalbumin (OVA)-induced allergic asthma model. OVA-challenged BALB/c mice were orally administrated with LLHsp65 for 10 consecutive days. The results demonstrate that LLhsp65 attenuates critical features of allergic inflammation, like airway hyperresponsiveness and mucus production. Likewise, the treatment decreases the pulmonary eosinophilia and the serum level of OVA-specific IgE. In addition to deviating immune responses towards Th1-cytokine profile, increase regulatory T cells, and cytokine levels, such as IL-6 and IL-10. Conclusions Our results reveal that the mucosal immunotherapy of LLHsp65 significantly reduces the overall burden of airway allergic inflammation, suggesting a promising therapeutic strategy for allergic asthma treatment. Significance and impact of the study This research reveals new perspectives on nonspecific immunotherapy based on the delivery of recombinant proteins by lactic acid bacteria to treat of allergic disorders. |
| Databáze: | OpenAIRE |
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