Detection of primary clarithromycin resistance of Helicobacter pylori and association between cagA + status and clinical outcome
| Autor: | Melek Inci, Murat Celik, Erkan Yula, Togrul Nagiyev, Fatih Köksal, Özlem Aycan Kaya |
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| Přispěvatelé: | Çukurova Üniversitesi |
| Rok vydání: | 2012 |
| Předmět: |
Adult
DNA Bacterial Male Rural Population medicine.medical_specialty Turkey Virulence Factors Atrophic gastritis Biopsy Microbial Sensitivity Tests Polymerase Chain Reaction Microbiology Gastroenterology Helicobacter Infections Agar dilution Young Adult Bacterial Proteins Duodenitis Clarithromycin Internal medicine Drug Resistance Bacterial Genotype Humans Medicine CagA Antigens Bacterial Helicobacter pylori biology business.industry General Medicine Middle Aged bacterial infections and mycoses medicine.disease biology.organism_classification Anti-Bacterial Agents Treatment Outcome Gastric Mucosa Immunology Female Gastritis medicine.symptom business Polymorphism Restriction Fragment Length medicine.drug |
| Zdroj: | Folia Microbiologica. 58:141-146 |
| ISSN: | 1874-9356 0015-5632 |
| DOI: | 10.1007/s12223-012-0192-8 |
| Popis: | PubMedID: 22956464 Helicobacter pylori was examined in 110 patients (82 (74.5) with gastritis, 18 (16.4) with duodenitis, six (5.5) with duodenal ulcer and gastroesophageal reflux, and four (3.6 %) with normal) with gastrointestinal problems living in rural area, no history of macrolide use, and detected by culture (71.8) or direct detection from gastric biopsies by PCR (82.7 %). Also, cagA gene was identified using PCR and was found positive in 68/91 (74.7 %) strains. The prevalence of clarithromycin-resistant H. pylori was investigated by two methods including PCR-RFLP (7.7 (A2142G 1.1 and A2143G 6.6 %)) and twofold agar dilution (8.9 %) to detect phenotypic and genotypic status simultaneously. Among all the H. pylori positive patients, eight (8.8 %) isolates were found to be resistant to clarithromycin by at least one of the AD and/or PCR-RFLP methods. H. pylori positive rates were significantly correlated with patients' sex, age, and endoscopic findings (p = 0.040 |
| Databáze: | OpenAIRE |
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