Hepatic induction of cholesterol biosynthesis reflects a remote adaptive response to pneumococcal pneumonia
| Autor: | Michael Kiehntopf, James C. Paton, Michael Bauer, Ulrich A. Maus, Nadine Ding, Martina Weber, Ralf A. Claus, David Enot, Abiodun D. Ogunniyi, Emeka I. Igwe, Debra Weih, Yoram Vodovotz, Hans P. Deigner, Sandro Lambeck, Lucien Frappart, David E. Briles, Matthias Kohl, Stefanie Henken, Thomas Kamradt |
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| Přispěvatelé: | Weber, Martina, Lambeck, Sandro, Ding, Nadine, Henken, Stefanie, Kohl, Matthias, Deigner, Hans P, Enot, David P, Igwe, Emeka I, Frappart, Lucien, Kiehntopf, Michael, Claus, Ralf A, Kamradt, Thomas, Weih, Debra, Vodovotz, Yoram, Briles, David E, Ogunniyi, Abiodun D, Paton, James C, Maus, Ulrich A, Bauer, Michael |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Life Sciences & Biomedicine - Other Topics
Biochemistry & Molecular Biology Protein Array Analysis Medizin Biology medicine.disease_cause Biochemistry Microbiology Sepsis sepsis Mice Bacterial Proteins Streptococcus pneumoniae Macrophages Alveolar Genetics medicine Animals Molecular Biology Pneumolysin Septic shock Cell Biology pneumolysin respiratory system Pneumonia Pneumococcal medicine.disease Mice Inbred C57BL Pneumonia Cholesterol Liver Pneumococcal pneumonia Immunology Lobar pneumonia Streptolysins Alveolar macrophage pathogen-host interaction Female Biotechnology |
| Popis: | Community-acquired pneumonia presents a spectrum of clinical phenotypes, from lobar pneumonia to septic shock, while mechanisms underlying progression are incompletely understood. In a transcriptomic and metabolomic study across tissues, we examined serotype-specific regulation of signaling and metabolic pathways in C57BL/6 mice intratracheally instilled with either serotype 19F Streptococcus pneumoniae (S19; causing lobar pneumonia), or serotype 2 S. pneumoniae (S2; causing septic pneumococcal disease,) or vehicle (Todd-Hewitt broth). Samples of lung, liver, and blood were collected at 6 and 24 h postinfection and subjected to microarray analysis and mass spectrometry. Results comprise a preferential induction of cholesterol biosynthesis in lobar pneumonia at low-infection doses (105 colony forming units/mouse) leading to increased plasma cholesterol (vehicle: 1.8 +/- 0.12 mM, S2: 2.3 +/- 0.10 mM, S19: 2.9 +/- 0.15 mM; P |
| Databáze: | OpenAIRE |
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