Alternative Transcription of Peroxisome Proliferator-Activated Receptor Gamma in the Liver Is Associated with Fatness of Chickens
| Autor: | R. Parada, Tadeusz Malewski, M. Kawka, Kazimierz Jaszczak |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Candidate gene medicine.medical_specialty Peroxisome proliferator-activated receptor gamma slow-growing chickens Peroxisome proliferator-activated receptor SNP Single-nucleotide polymorphism Biology Fat deposition 03 medical and health sciences Transcription (biology) Internal medicine Gene expression lcsh:Zoology medicine lcsh:QL1-991 Receptor Transcription factor transcription factor lcsh:SF1-1100 chemistry.chemical_classification lcsh:Veterinary medicine 030104 developmental biology Endocrinology chemistry PPARG lcsh:SF600-1100 Animal Science and Zoology lcsh:Animal culture |
| Zdroj: | Brazilian Journal of Poultry Science v.20 n.3 2018 Brazilian Journal of Poultry Science Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) instacron:FACTA Brazilian Journal of Poultry Science, Vol 20, Iss 3, Pp 447-454 |
| ISSN: | 0152-9293 |
| Popis: | The expression of four transcription variant of peroxisome proliferator-activated receptor gamma gene (PPARG) (XM_015292931.1; XM_015292932.1; XM_015292933.1 and NM_001001460.1) in the liver of broilers was measured and its correlation with abdominal fat weight and relative abdominal fat content was investigated. The study was conducted with 92 slow-growing crossbred chickens (Cobb males x indigenous Green-legged Partridge female chickens) divided into “fat” and “lean” groups, according to their abdominal fat yield. The NM_001001460.1 transcriptwas upregulated with ratio of means 4.26 (p≤0.01) in the “fat” group in relation to the “lean” group. Expression of this transcript was highly correlated with relative abdominal fat content (0.71, p≤0.01) and abdominal fat weight (0.59, p≤0.01). Two SNPs are located in putative transcription factor binding sites. Mutation -991C>A disrupts PPAR while mutation -884C>T disrupts C/EBP putative binding site. The gene expression analysis of PPARg showed that the expression of the transcripts (NM_001001460.1) was more than four times higher in fat than in lean chickens. These results point out that the peroxisome proliferator-activated receptor gamma NM_001001460.1 transcript could be candidate gene for determination of abdominal fat deposition in the chickens. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|