Radiosensitization of non-small cell lung carcinoma by EGFR inhibition
| Autor: | Giuseppe Privitera, M Tanja Bulat, D Otilija Keta, J Jelena Zakula, M Aleksandra Ristic-Fira, B Lela Koricanac, Giacomo Cuttone, M Ivan Petrovic |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Oncology
erlotinib medicine.medical_specialty γ-ray DNA repair Cell human lung adenocarcinoma cell Internal medicine medicine lcsh:Nuclear and particle physics. Atomic energy. Radioactivity gamma-ray Radiosensitivity Viability assay Epidermal growth factor receptor Safety Risk Reliability and Quality hu man lung adenocarcinoma cell g-ray DNA dam age radiosensitization biology Chemistry medicine.disease 3. Good health medicine.anatomical_structure Nuclear Energy and Engineering Cancer research biology.protein lcsh:QC770-798 DNA damage Adenocarcinoma Erlotinib Tyrosine kinase medicine.drug |
| Zdroj: | Nuclear technology and radiation protection Nuclear Technology and Radiation Protection, Vol 29, Iss 3, Pp 233-241 (2014) |
| ISSN: | 1452-8185 1451-3994 |
| DOI: | 10.2298/ntrp1403233k |
| Popis: | Molecular targeted cancer therapy is a promising treatment strategy. Considering the central role of the epidermal growth factor receptor in cell proliferation and survival, there are indications that targeted agents like tyrosine kinase inhibitors, i. e., erlotinib, may enhance the antitumor treatment by radiation. The aim of this study is to analyze the inactivation effects of g-rays and to test the radiosensitizing potential of erlotinib on human lung adenocarcinoma cells in vitro. Irradiations were performed with doses ranging from 1 Gy to 8 Gy. In order to increase the radiosensitivity of CRL-5876 lung adenocarcinoma cells, the cells were treated with a clinically relevant concentration of 2 µM erlotinib. The effects of single and combined treatments were monitored using clonogenic survival, cell viability and proliferation assays at different time points. For the detection and visualization of the phosphorylated histone H2AX (γ-H2AX), an important biological marker of DNA double-strand break formation, fluorescence immunocytochemistry, was performed. The response to the treatment was monitored at four time points: 30 min, 2, 6, and 24 h. Irradiations with g-rays resulted in significant cell inactivation regarding all analyzed biological endpoints. Combined treatments revealed consistent cell inactivation. Moreover, compared to g-rays alone, elevated levels of g-H2AX foci were observed after pretreatment with erlotinib, indicating radiosensitization through impaired DNA repair. [Projekat Ministarstva nauke Republike Srbije, br. 173046 i br. 171019] |
| Databáze: | OpenAIRE |
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