Effect of photodynamic therapy on tumor necrosis factor production by murine macrophages
| Autor: | Steven Evans, Douglas L. Fraker, Jeffrey A. Norton, Wilbert Matthews, Roger R. Perry, Havey I. Pass |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Lipopolysaccharides
Cancer Research Pathology medicine.medical_specialty Necrosis Cell Survival medicine.medical_treatment Photodynamic therapy Hemorrhage Biology Mice Tumor necrosis factor production In vivo medicine Macrophage Animals Cytotoxicity Tumor Necrosis Factor-alpha Macrophages Mice Inbred C57BL Cytokine Oncology Photochemotherapy Cancer research Blood Vessels Tumor necrosis factor alpha Female medicine.symptom |
| Zdroj: | Journal of the National Cancer Institute. 82(1) |
| ISSN: | 0027-8874 |
| Popis: | Photodynamic therapy (PDT) involves the treatment of tumors in the presence of sensitizer, light, and oxygen, causing energy-dependent cytotoxicity. A vascular effect that causes hemorrhagic tumor necrosis has been described with PDT, but its basis remains undefined. To investigate the possible role of tumor necrosis factor (TNF) production in the generation of such a vascular effect and/or a direct tumor effect, we treated thioglycollate-elicited murine macrophages with PDT, and we measured the possible production of TNF using the L929 assay. An energy-dependent production of TNF by macrophage treated with PDT, stimulated or unstimulated with endotoxin, was demonstrated, and TNF production was inhibited at the highest treatment energy levels. These data represent the first description of cytokine production by PDT-treated macrophages, and may serve as another mechanism of PDT cytotoxicity in vivo, either directly by TNF-mediated tumor necrosis, or indirectly by vascular effects on tumor vessels. |
| Databáze: | OpenAIRE |
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