Identification of a point mutation in the ace1gene ofTherioaphis trifollimaculata and detection of insecticide resistance by a diagnostic PCR–RFLP assay
| Autor: | J.T. Margaritopoulos, John Vontas, E. AlSuhaibani, A. Kotzamumin, R. Al-Atiyat, Costas Ch. Voudouris |
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| Rok vydání: | 2015 |
| Předmět: |
DNA Mutational Analysis
medicine.disease_cause Polymerase Chain Reaction Insecticide Resistance Sequence Analysis Protein Botany medicine Animals Point Mutation Genetics Aphid Mutation biology Point mutation food and beverages Aphididae General Medicine biology.organism_classification Resistance mutation Hemiptera Restriction site Aphids Insect Science Acetylcholinesterase Insect Proteins Restriction fragment length polymorphism Agronomy and Crop Science Polymorphism Restriction Fragment Length |
| Zdroj: | Bulletin of Entomological Research. 105:712-716 |
| ISSN: | 1475-2670 0007-4853 |
| DOI: | 10.1017/s0007485315000668 |
| Popis: | Aphids are important agricultural pests worldwide. Their control is largely based on chemical insecticides. One species that shows important invasive abilities and host-plant-related differences isTherioaphis trifolii(Monell) (Hemiptera: Aphididae).T. trifolii maculata, also known as spotted alfalfa aphid (SAA), can be very injurious to alfalfa crops in certain regions, such as in Saudi Arabia for effective control it is essential to diagnose and monitor the resistance mechanisms in the SAA populations. In the present study, we analysed acetylcholinesterase (ace) target site insensitivity mechanisms. A 650 bp length DNA containing the putative acetylcholinesterase (ace1) precursor was obtained and compared with other Hemipteran species. The sequences of many individual aphids collected from alfalfa crops in Saudi Arabia were analysed for the presence of resistance mutations: no resistance mutations were found at the resistance mutation loci 302; however, the presence of a serine–phenylalanine substitution (S431F) was identified in one individual. The S431F substitution, has been shown to confer significant levels of both organophosphate and carbamate resistance in other aphid species, and is now found for the first time inT. trifolii. We subsequently developed a simple polymerase chain reaction–restriction fragment length polymorphism assays for the S431F mutation, using a TaqI restriction site destroyed by the S431F mutation. The novel diagnostic assay may support the implementation of Insecticide Resistance Management strategies, for the control of SAA in alfalfa crops in the Kingdom of Saudi Arabia, and other countries worldwide. |
| Databáze: | OpenAIRE |
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