A t(11;15) fuses MLL to two different genes, AF15q14 and a novel gene MPFYVE on chromosome 15
| Autor: | Janet D. Rowley, María D. Odero, Andy S. Chien, Nancy J. Zeleznik-Le, Vandana Chinwalla, Mary Beth Neilly |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Cancer Research DNA Complementary Oncogene Proteins Fusion Acute myeloblastic leukemia Amino Acid Motifs Molecular Sequence Data Chromosomal translocation Biology Translocation Genetic Chromosome 15 hemic and lymphatic diseases Genetics medicine Humans Amino Acid Sequence Child neoplasms Molecular Biology Gene In Situ Hybridization Fluorescence Expressed Sequence Tags Oncogene Proteins Chromosomes Human Pair 15 Base Sequence Sequence Homology Amino Acid Chromosomes Human Pair 11 Proteins medicine.disease Fusion protein Molecular biology Protein Structure Tertiary Leukemia Myeloid Acute Leukemia Fusion transcript FYVE domain Sequence Alignment Myeloid-Lymphoid Leukemia Protein |
| Zdroj: | Oncogene. 22:1400-1410 |
| ISSN: | 1476-5594 0950-9232 |
| Popis: | The mixed lineage leukemia gene (MLL, also known as HRX, ALL-1 and Htrx) located at 11q23 is involved in translocations with over 40 different chromosomal bands in a variety of leukemia subtypes. Here we report our analysis of a rare but recurring translocation, t(11;15)(q23;q14). This translocation has been described in a small subset of cases with both acute myeloblastic leukemia and ALL. Recent studies have shown that MLL is fused to AF15q14 in the t(11;15). Here we analyse a sample from another patient with this translocation and confirm the presence of an MLL-AF15q14 fusion. However, we have also identified and cloned another fusion transcript from the same patient sample. In this fusion transcript, MLL is fused to a novel gene, MLL partner containing FYVE domain (MPFYVE). Both MLL-AF15q14 and MLL-MPFYVE are in-frame fusion transcripts with the potential to code for novel fusion proteins. MPFYVE is also located on chromosome 15, approximately 170 kb telomeric to AF15q14. MPFYVE contains a highly conserved motif, the FYVE domain which, in other proteins, has been shown to bind to phosphotidyl-inositol-3 phosphate (PtdIns(3)P). The MLL-MPFYVE fusion may be functionally important in the leukemia process in at least some patients containing this translocation. |
| Databáze: | OpenAIRE |
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