Quantitative proteomic Analysis Reveals up-regulation of caveolin-1 in FOXP3-overexpressed human gastric cancer cells
| Autor: | Jingjing Lian, Xiaoqing Zeng, Shiyao Chen, Xiaoquan Huang, Qing Miao, Na Li, Xuanling Du, Jing Gao, Duyi Pan, Li-Li Xu, Hu Zhou, Gui-Fen Ma |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Proteomics Transcriptional Activation Caveolin 1 lcsh:Medicine Article 03 medical and health sciences Cell Movement Stomach Neoplasms Caveolae Cell Line Tumor Transcriptional regulation Humans Neoplasm Invasiveness lcsh:Science Cell Proliferation Multidisciplinary Chemistry lcsh:R Stomach Proteins Cell migration Forkhead Transcription Factors Prognosis Cell biology Up-Regulation 030104 developmental biology Tumor progression Cancer cell Apoptotic signaling pathway lcsh:Q Signal transduction Signal Transduction |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
| ISSN: | 2045-2322 |
| Popis: | Forkhead box protein 3 (FOXP3) is implicated in tumor progression and prognosis in various types of tumor cells. We have recently reported that FOXP3 inhibited proliferation of gastric cancer (GC) cells through activating the apoptotic signaling pathway. In this study, we found that over-expression of FOXP3 inhibited GC cell migration, invasion and proliferation. Then, the label-free quantitative proteomic approach was employed to further investigating the down-stream proteins regulated by FOXP3, resulting in a total of 3,978 proteins quantified, including 186 significantly changed proteins. Caveolin-1 (CAV1), as a main constituent protein of caveolae, was one of those changed proteins up-regulated in FOXP3-overexpressed GC cells, moreover, it was assigned as one of the node proteins in the protein-protein interaction network and the key protein involved in focal adhesion pathway by bioinformatics analysis. Further biological experiments confirmed that FOXP3 directly bound to the promoter regions of CAV1 to positively regulate CAV1 transcription in GC cells. In summary, our study suggested that FOXP3 can be considered as a tumor suppressor in GC via positively regulating CAV1 through transcriptional activation, and this FOXP3-CAV1 transcriptional regulation axis may play an important role in inhibiting invasion and metastasis of GC cells. Data are available via ProteomeXchange under identifier PXD007725. |
| Databáze: | OpenAIRE |
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