The Drosophila SDE3 Homolog armitage Is Required for oskar mRNA Silencing and Embryonic Axis Specification
| Autor: | Birgit S. Koppetsch, William E. Theurkauf, Heather A Cook, Jing Wu |
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| Rok vydání: | 2004 |
| Předmět: |
DNA
Complementary Embryo Nonmammalian Molecular Sequence Data Biology oskar Microtubules General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine RNA interference Animals Drosophila Proteins Gene silencing Amino Acid Sequence Cytoskeleton Body Patterning 030304 developmental biology 0303 health sciences Messenger RNA Base Sequence Arabidopsis Proteins Biochemistry Genetics and Molecular Biology(all) urogenital system Cell Polarity Embryonic axis specification RNA Helicase A Molecular biology Cell biology RNA silencing Drosophila melanogaster Protein Biosynthesis Mutation Oocytes Female RNA Interference RNA Helicases 030217 neurology & neurosurgery |
| Zdroj: | Cell. 116:817-829 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/s0092-8674(04)00250-8 |
| Popis: | Polarization of the microtubule cytoskeleton during early oogenesis is required to specify the posterior of the Drosophila oocyte, which is essential for asymmetric mRNA localization during mid-oogenesis and for embryonic axis specification. The posterior determinant oskar mRNA is translationally silent until mid-oogenesis. We show that mutations in armitage and three components of the RNAi pathway disrupt oskar mRNA translational silencing, polarization of the microtubule cytoskeleton, and posterior localization of oskar mRNA. armitage encodes a homolog of SDE3, a presumptive RNA helicase involved in posttranscriptional gene silencing (RNAi) in Arabidopsis, and is required for RNAi in Drosophila ovaries. Armitage forms an asymmetric network associated with the polarized microtubule cytoskeleton and is concentrated with translationally silent oskar mRNA in the oocyte. We conclude that RNA silencing is essential for establishment of the cytoskeletal polarity that initiates embryonic axis specification and for translational control of oskar mRNA. |
| Databáze: | OpenAIRE |
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