The Receptor Binding Domain of the New Middle East Respiratory Syndrome Coronavirus Maps to a 231-Residue Region in the Spike Protein That Efficiently Elicits Neutralizing Antibodies
| Autor: | Mou, H., Stalin Raj, V., van Kuppeveld, F.J.M., Rottier, P.J.M., Haagmans, B.L., Bosch, B.J., Strategic Infection Biology, Dep Infectieziekten Immunologie, Faculteit Diergeneeskunde, I&I SIB1 |
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| Přispěvatelé: | Strategic Infection Biology, Dep Infectieziekten Immunologie, Faculteit Diergeneeskunde, I&I SIB1, Virology, Medical Oncology |
| Rok vydání: | 2013 |
| Předmět: |
Middle East respiratory syndrome coronavirus
Dipeptidyl Peptidase 4 Immunology Coronacrisis-Taverne Virus Attachment Antibodies Viral medicine.disease_cause Microbiology Antibodies Epitope Cell Line Epitopes 03 medical and health sciences Viral Envelope Proteins Virology Receptors medicine Humans Viral Binding site Receptor Neutralizing Dipeptidyl peptidase-4 030304 developmental biology Coronavirus 0303 health sciences Binding Sites Membrane Glycoproteins biology 030306 microbiology B-Lymphocyte Antibodies Neutralizing Molecular biology Spike Glycoprotein Virus Virus-Cell Interactions 3. Good health Cell culture Insect Science Spike Glycoprotein Coronavirus biology.protein Epitopes B-Lymphocyte Receptors Virus Antibody |
| Zdroj: | Journal of Virology, 87(16), 9379. American Society for Microbiology Journal of Virology; Vol 87 Journal of Virology, 87(16), 9379-9383. American Society for Microbiology |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/JVI.01277-13 |
| Popis: | The spike (S) protein of the recently emerged human Middle East respiratory syndrome coronavirus (MERS-CoV) mediates infection by binding to the cellular receptor dipeptidyl peptidase 4 (DPP4). Here we mapped the receptor binding domain in the S protein to a 231-amino-acid fragment (residues 358 to 588) by evaluating the interaction of spike truncation variants with receptor-expressing cells and soluble DPP4. Antibodies to this domain—much less so those to the preceding N-terminal region—efficiently neutralize MERS-CoV infection. |
| Databáze: | OpenAIRE |
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