Transcription Factors GATA-4 and GATA-6 and a GATA Family Cofactor, FOG-2, Are Expressed in Human Ovary and Sex Cord-Derived Ovarian Tumors*
| Autor: | Ilkka Ketola, Ralf Bützow, Olli Ritvos, Mika Laitinen, David B. Wilson, Mikko Anttonen, Markku Heikinheimo |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Endocrinology
Diabetes and Metabolism Clinical Biochemistry Biochemistry 0302 clinical medicine Endocrinology GATA6 Transcription Factor RNA Neoplasm Cloning Molecular Cells Cultured In Situ Hybridization Ovarian Neoplasms 0303 health sciences Reverse Transcriptase Polymerase Chain Reaction Theca Cell Zinc Fingers Middle Aged Immunohistochemistry 3. Good health DNA-Binding Proteins Gene Expression Regulation Neoplastic Blotting Southern medicine.anatomical_structure Theca 030220 oncology & carcinogenesis embryonic structures Female Folliculogenesis DNA Probes Adult endocrine system medicine.medical_specialty Ovary In situ hybridization Biology 03 medical and health sciences Corpus Luteum Internal medicine medicine Humans Sex Cord-Gonadal Stromal Tumors Ovarian follicle Aged 030304 developmental biology Granulosa Cells urogenital system Biochemistry (medical) Antral follicle GATA4 Transcription Factor Cell culture Transcription Factors |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 85:3476-3483 |
| ISSN: | 1945-7197 0021-972X |
| Popis: | Previous studies have implicated transcription factors GATA-4 and GATA-6 in the regulation of murine ovarian development and function. In rodents, GATA-4 is expressed in granulosa cells of primary and early antral follicles, whereas GATA-6 is expressed in granulosa cells of late antral follicles and luteal glands. Both transcription factors can be detected in lesser amounts in theca cells and interstitial cells. We have now examined the expression of GATA-4 and GATA-6 in human ovaries, human granulosa-luteal (GL) cells and sex cord-derived tumors. We show by in situ hybridization and immunohistochemistry that GATA-4 and GATA-6 messenger RNA (mRNA) and GATA-4 protein are present in granulosa and theca cells in both preantral and antral follicles. Both human ovarian tissue samples and freshly isolated GL cells derived from preovulatory follicles of gonadotropin-treated women express GATA-4, GATA-6, and FOG-2 transcripts, and GATA-6 mRNA expression in GL cell cultures is stimulated by human CG and 8-bromo-cAMP. The vast majority of granulosa and theca cell tumors examined expressed GATA-4 and GATA-6. We also found that mRNA for FOG-2, a recently discovered regulator of GATA-4, is coexpressed with GATA-4 in human ovary samples, normal granulosa cells, and in sex cord-derived tumors. Our results demonstrate that GATA-4, GATA-6, and FOG-2 are expressed in human ovary and in granulosa and theca cell tumors. Our findings support a role for GATA-binding proteins in human ovarian folliculogenesis. Moreover, these data suggest that GATA factors may contribute to the phenotypes of sex cord-derived ovarian tumors. |
| Databáze: | OpenAIRE |
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