Evaluation of in vitro solar protection factor (SPF), antioxidant activity, and cell viability of mixed vegetable extracts from Dirmophandra mollis Benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L
| Autor: | Ana R. Fernandes, Mary Ann Foglio, Elena Sánchez-López, Priscila Gava Mazzola, Letícia Caramori Cefali, Ilza Maria de Oliveira Sousa, Eliana B. Souto, Mariana Cecchetto Figueiredo, Janaína Artem Ataide, Ana Lúcia Tasca Gois Ruiz |
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| Přispěvatelé: | Universidade do Minho |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Antioxidant antioxidant Sunscreens (Cosmetics) DPPH Ruta graveolens medicine.medical_treatment Plant Science Antioxidants quercetin 030207 dermatology & venereal diseases 03 medical and health sciences Rutin chemistry.chemical_compound 0302 clinical medicine medicine Viability assay Flavonoides Ecology Evolution Behavior and Systematics cell viability Flavonoids ABTS sun protection factor Science & Technology Ecology biology Traditional medicine Chemistry Ginkgo biloba Protectors solars rutin Botany biology.organism_classification 030104 developmental biology QK1-989 flavonoids Quercetin |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Plants Volume 8 Issue 11 Plants, Vol 8, Iss 11, p 453 (2019) |
| Popis: | The aim of this study was to validate a HPLC method for the assay of flavonoids in extracts obtained from natural sources, i.e., from Dirmophandra mollis Benth, Ginkgo biloba L., Ruta graveolens L., and Vitis viní fera L. The potential sun protecting effect, antioxidant activity, and cell viability of the extracts were also determined. Individual extracts (obtained from each individual species) and a mixed extract (containing the four extracts) were analyzed by the validated HPLC method for the identification of flavonoids and quantification of rutin and quercetin. An in vitro cell viability study was carried out using the neutral red method. The in vitro sun protection factor was determined by spectral transmittance and in vitro antioxidant efficacy was evaluated against DPPH, ABTS, and AAPH radicals. The HPLC method used for the identification and quantification of flavonoids in extracts exhibited linearity, precision, accuracy, and robustness. Detection and quantification limits were, respectively, 2.881 ± 0.9 &mu g· mL&minus 1 and 0.864 ± 1 for quercetin, and 30.09 ± 1 &mu 1 and 9.027 ± 1.1 &mu 1 for rutin. All extracts did not affect cell viability at the evaluated concentration range and exhibited a sun protection effect and antioxidant activity. Among the evaluated extracts, Ginkgo biloba L. and the mixed extract depicted the most expressive antioxidant activity. The mixed extract exhibited sunscreen protection against ultraviolet A (UVA) and ultraviolet B (UVB) and a critical wavelength of 372.7 ± 0.1. Our results translate the enhanced flavonoids&rsquo composition of the mixed extract, which may be a potential alternative over sunscreens and antioxidants in pharmaceutic/cosmetic formulations. |
| Databáze: | OpenAIRE |
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