Reliable reference gene selection for quantitative real time PCR in Haemonchus contortus
| Autor: | Lenka Lecová, Lukáš Prchal, Michaela Růžičková, Jiří Lamka, Lenka Skálová, Petra Matoušková, Heiko Vogel, Ivan Vokřál, Rosalind Laing, Barbora Szotáková |
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| Rok vydání: | 2015 |
| Předmět: |
Anthelmintics
Genetics Candidate gene Gene Expression Profiling Reference Standards Biology Real-Time Polymerase Chain Reaction biology.organism_classification Gene expression profiling Real-time polymerase chain reaction Reference genes parasitic diseases Animals Reference gene Haemonchus Parasitology Molecular Biology Gene Selection (genetic algorithm) Haemonchus contortus |
| Zdroj: | Molecular and Biochemical Parasitology |
| ISSN: | 0166-6851 |
| Popis: | The aim of this work was to identify reliable reference genes for expression studies in adult Haemonchus contortus. Eleven candidate genes were identified and the stability of their expression was assessed in adult males and females of two genetically divergent H. contortus isolates: drug-susceptible (ISE) and multi-drug-resistant (WR). Five genes with the most stable expression patterns were further assessed for suitability as reference genes in anthelmintic-treated H. contortus adults versus non-treated controls. We identified important differences in the expression of a number of candidate genes in anthelmintic-treated samples, confirming the need for careful validation of control genes for such experiments. We propose the use of multiple reference genes for expression studies in this species and found gpd, ama and far most suitable for adult H. contortus. |
| Databáze: | OpenAIRE |
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