Identification of the Amipurimycin Gene Cluster Yields Insight into the Biosynthesis of C9 Sugar Nucleoside Antibiotics
| Autor: | Hai-Xue Pan, Wen-Jia Kang, Gong-Li Tang, Shengyang Wang, Biao Yu, Hui-Ming Hua |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Carboxylic acid
Mutant 010402 general chemistry medicine.disease_cause 01 natural sciences Biochemistry chemistry.chemical_compound Adenosine Triphosphate Biosynthesis Gene cluster medicine Escherichia coli Cycloleucine Physical and Theoretical Chemistry chemistry.chemical_classification Mutation 010405 organic chemistry Chemistry Organic Chemistry Nucleosides In vitro Streptomyces 0104 chemical sciences Anti-Bacterial Agents Biosynthetic Pathways Enzymes Enzyme Purines Multigene Family Sugars Nucleoside |
| Zdroj: | Organic letters. 21(9) |
| ISSN: | 1523-7052 |
| Popis: | Feeding studies indicate a possible synthetic pattern for the N-terminal cis-aminocyclopentane carboxylic acid (ACPC) and suggest an unusual source of the high-carbon sugar skeleton of amipurimycin (APM). The biosynthetic gene cluster of APM was identified and confirmed by in vivo experiments. A C9 core intermediate was discovered from null mutants of ACPC pathway, and an ATP-grasp enzyme (ApmA8) was reconstituted in vitro for ACPC loading. Our observations allow a first proposal of the APM biosynthetic pathway. |
| Databáze: | OpenAIRE |
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