Antioxidant effects of antioxidant biofactor on reactive oxygen species in human gingival fibroblasts
| Autor: | Yasuhisa Tsujimoto, Kiyoshi Matsushima, Satoshi Matsui, Toshihiko Ozawa |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
free radical
collagen chemistry.chemical_classification Reactive oxygen species Nutrition and Dietetics Antioxidant medicine.medical_treatment Radical Clinical Biochemistry electron spin resonance spin-trapping method periodontal disease Medicine (miscellaneous) antioxidant baiofactor medicine.disease_cause chemistry.chemical_compound Biochemistry Periodontal disease chemistry Superoxide radical medicine Original Article Hydroxyl radical Type I collagen Oxidative stress |
| Zdroj: | Journal of Clinical Biochemistry and Nutrition |
| ISSN: | 1880-5086 0912-0009 |
| Popis: | The purpose of this study was to investigate the effects of antioxidant biofactor (AOB) on reactive oxygen species (ROS). Generation of superoxide radical (O(2) (•-)) and hydroxyl radical ((•)OH) was determined using an electron spin resonance (ESR) spin-trapping method. AOB was added at different concentrations to these free radical generating systems. The generation of both O(2) (•-) and (•)OH was scavenged by the addition of AOB in a dose-dependent manner. These results indicate that AOB has strong antioxidant properties against these radicals. We further investigated the anti-oxidative effect of AOB on human gingival fibroblasts (HGFs). HGFs were treated for 3 h with α-MEM containing a combination of AOB and H(2)O(2) (AOB + H(2)O(2) group), containing H(2)O(2) (H(2)O(2) group), or containing AOB alone (AOB group). Non-stimulated HGFs were used as a control group. The number of surviving cells was in the order of the AOB groupcontrol groupAOB + H(2)O(2) groupH(2)O(2) group. The level of expression of type I collagen mRNA and production of collagen were also in the order of the AOB groupcontrol groupAOB + H(2)O(2) groupH(2)O(2) group. In conclusion, our results suggest that AOB may protect HGFs against oxidative stress by reducing stress-induced ROS. |
| Databáze: | OpenAIRE |
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