Interleukin-16 Inhibits Human Immunodeficiency Virus Type 1 Entry and Replication in Macrophages and in Dendritic Cells
| Autor: | George M. Bahr, Joëlle Dewulf, Emmanuel Hermann, Edith Darcissac, André Capron, Marie-José Truong |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_treatment
Immunology Gene Expression chemical and pharmacologic phenomena Biology Virus Replication Microbiology law.invention Proviruses law Viral entry Virology medicine Humans RNA Messenger Chemokine CCL4 Macrophage inflammatory protein Interleukin-16 Macrophages Dendritic Cells Macrophage Inflammatory Proteins Recombinant Proteins Reverse transcriptase Cytokine Viral replication Cell culture Insect Science DNA Viral HIV-1 Recombinant DNA Cytokines RNA Viral Pathogenesis and Immunity Interleukin 16 |
| Zdroj: | Journal of Virology. 73:7008-7013 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Recombinant interleukin-16 (rIL-16) has been found to inhibit human immunodeficiency virus type 1 (HIV-1) replication in acutely or endogenously infected CD4 + T cells. However, the effect of rIL-16 on HIV-1 replication in antigen-presenting cells (APCs) is still unknown. We show here a potent HIV-suppressive activity of rIL-16 in acutely infected monocyte-derived macrophages and dendritic cells determined by the levels of viral RNA transcripts or of viral reverse transcriptase in culture supernatants. The observed effect was dependent on the presence of rIL-16 early after infection and could not be induced by a 24-h treatment of cells with the cytokine prior to infection. Using macrophage-tropic and dually tropic primary isolates, we also showed that the addition of rIL-16 to cell cultures only during the infection period was effective in blocking virus entry and reducing proviral DNA levels in APCs. However, the anti-HIV activity of rIL-16 could not be linked to the induction of virus-suppressive concentrations of β-chemokines or to the inhibition of HIV-enhancing cytokines. These findings establish a critical role for rIL-16 in protecting APCs against HIV-1 infection and lend further support to its potential use in the treatment of HIV disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|