Breakdown of cell-collagen networks through collagen remodeling
| Autor: | Anaïs Gérard, Claude Verdier, Alain Duperray, Andreea Iordan, Alexei Grichine |
|---|---|
| Přispěvatelé: | Laboratoire de Spectrométrie Physique (LSP), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), INSERM U823, équipe 8 (Immunologie Analytique des Pathologies Chroniques), Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Marie Curie Research Training Network (MRTN-CT-2004-503661), Verdier, Claude |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
collagen
reflectance Physiology Confocal Cell CHO Cells law.invention Cricetulus Cell Movement Confocal microscopy law Cricetinae Physiology (medical) [SPI.MECA.BIOM] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph] Collagen network Cell Adhesion medicine Animals [PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph] Cell Shape remodeling Microscopy Confocal Chemistry [PHYS.MECA.BIOM] Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph] [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph] tissue Anatomy Reflectivity Elasticity Extracellular Matrix medicine.anatomical_structure confocal Biophysics rheology Stress Mechanical Gels |
| Zdroj: | Biorheology Biorheology, 2010, 47 (5-6), pp.277-295. ⟨10.3233/BIR-2010-0575⟩ |
| ISSN: | 0006-355X |
| DOI: | 10.3233/BIR-2010-0575⟩ |
| Popis: | International audience; Collagen model tissues are analyzed, which consist of cells embedded in a collagen matrix at different concentrations (of cells and collagen). Rheological properties are measured and complementary confocal microscopy analyses are carried out. An important feature is observed, corresponding to the breakdown of the collagen network (i.e. decrease in network elasticity) for high collagen concentrations, due to the presence of cells. Thanks to confocal microscopy, we show that cells elongate within the gel and can remodel it, this being a concentration-dependent feature. A careful analysis of the remodelling process shows that cells can attract collagen in their close neighborhood, this being an irreversible process, and that migrating cells create collagen-depleted regions behind them. |
| Databáze: | OpenAIRE |
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