Anti-allergic Inflammatory Effects of the Essential Oil From Fruits of Zanthoxylum coreanum Nakai
| Autor: | Young Ran Kim, Ji Yoon Yang, Jae Hun Ahn, Se Jin Jo, Mi Jin Park, Jong-Hwan Park, Jung Up Park, Sung Suk Lee, Rui Hong Guo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway p38 mitogen-activated protein kinases inflammatory mediators Pharmacology NF-κB law.invention 03 medical and health sciences chemistry.chemical_compound law Zanthoxylum coreanum Nakai Pharmacology (medical) Cytotoxicity Essential oil degranulation Zanthoxylum coreanum biology Chemistry lcsh:RM1-950 Degranulation COX-2 biology.organism_classification iNOS 030104 developmental biology lcsh:Therapeutics. Pharmacology Phosphorylation |
| Zdroj: | Frontiers in Pharmacology, Vol 9 (2018) |
| ISSN: | 1663-9812 |
| DOI: | 10.3389/fphar.2018.01441/full |
| Popis: | Zanthoxylum coreanum Nakai is a rare shrub which grows in Korea and China. Pericarp of Z. coreanum has been used as a crude medicine, but there are few researches about the pharmacologic activities. The present study was designed to investigate the anti-allergic inflammatory activities of the essential oil from fruits of Zanthoxylum coreanum Nakai (ZCO). Our findings showed that ZCO inhibited both the IgE-antigen complex or PMA/A23187-induced β-hexosaminidase release and IL-4 production dose-dependently in RBL-2H3 mast cells, and confirmed that ZCO at the tested concentrations did not show cytotoxicity to RBL-2H3 cells by MTS assay. Additionally, we found that ZCO showed the significant inhibition on LPS-induced overproduction of TNF-α, IL-6 and NO. Consistently, the protein levels of iNOS and COX-2 were also remarkably decreased by ZCO treatment. Herein, Our mechanistic studies revealed that ZCO significantly suppressed the activation of transcription factor NF-κB in PMA-activated 293T cells, and further inhibited NF-κB p65 translocation into the nucleus in LPS-stimulated RAW264.7 cells. Further investigation identified that ZCO down-regulated LPS-induced phosphorylation of MAPK (JNK, ERK, and p38) signal pathway. For incremental research, we established an DNCB-induced atopic dermatitis model in BALB/c mice, and found that ZCO remarkably inhibited DNCB-induced ear swelling and AD-like symptoms. Based on these findings, ZCO is suggested to have a therapeutic potential for the allergic inflammatory diseases. |
| Databáze: | OpenAIRE |
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