Skin transcriptome reveals the dynamic changes in the Wnt pathway during integument morphogenesis of chick embryos

Autor: Xue Bai, Husile Gong, Bin Liu, WangMei Qi, Wenguang Zhang, YueXing Wang, Hong Wang, JinFeng He, Jianghong Wu
Rok vydání: 2017
Předmět:
0301 basic medicine
lcsh:Medicine
Gene Expression
Chick Embryo
Poultry
Transcriptome
Cell Signaling
Gene expression
Morphogenesis
Medicine and Health Sciences
Gamefowl
Animal Anatomy
lcsh:Science
Wnt Signaling Pathway
WNT Signaling Cascade
Skin
Regulation of gene expression
Multidisciplinary
Wnt signaling pathway
Gene Expression Regulation
Developmental
Eukaryota
Genomics
Signaling Cascades
Cell biology
Multigene Family
Vertebrates
Integument
Anatomy
Integumentary System
Transcriptome Analysis
Research Article
Signal Transduction
animal structures
Signal Inhibition
Biology
Birds
03 medical and health sciences
Genetics
Animals
KEGG
lcsh:R
Organisms
Biology and Life Sciences
Computational Biology
Cell Biology
Feathers
Genome Analysis
030104 developmental biology
Fowl
Amniotes
lcsh:Q
Skin morphogenesis
Zoology
Chickens
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 1, p e0190933 (2018)
ISSN: 1932-6203
Popis: Avian species have a unique integument covered with feathers. Skin morphogenesis is a successive and complex process. To date, most studies have focused on a single developmental point or stage. Fewer studies have focused on whole transcriptomes based on the time-course of embryo integument development. To analyze the global changes in gene expression profiles, we sequenced the transcriptome of chicken embryo skin samples from day 6 to day 21 of incubation and identified 5830 differentially expressed genes (DEGs). Hierarchical clustering showed that E6 to E14 is the critical period of feather follicle morphogenesis. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the DEGs, two kinds of Wnt signaling pathways (a canonical pathway and a non-canonical pathway) changed during feather follicle and feather morphogenesis. The gene expression level of inhibitors and ligands related to the Wnt signaling pathway varied significantly during embryonic development. The results revealed a staggered phase relationship between the canonical pathway and the non-canonical pathway from E9 to E14. These analyses shed new light on the gene regulatory mechanism and provided fundamental data related to integument morphogenesis of chickens.
Databáze: OpenAIRE
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