Monoclonal anti-idiotype induces antibodies against bovine Q17 rotavirus
| Autor: | E. Cornaglia, B. G. Talbot, Youssef Elazhary, Bb. R. Brodeur |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Rotavirus
medicine.drug_class viruses Guinea Pigs Immunology Reoviridae Enzyme-Linked Immunosorbent Assay Antibodies Viral Monoclonal antibody medicine.disease_cause Microbiology Virus Neutralization Immunoglobulin Fab Fragments Mice Capsid Neutralization Tests Virology medicine Animals Neonatal Calf Diarrhea Virus Mice Inbred BALB C biology Antibodies Monoclonal virus diseases biology.organism_classification Antibodies Anti-Idiotypic Insect Science Monoclonal biology.protein Capsid Proteins Cattle Electrophoresis Polyacrylamide Gel Antibody Research Article |
| Zdroj: | Journal of Virology. 66:5763-5769 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.66.10.5763-5769.1992 |
| Popis: | This study describes, for the first time, the production and use of an "internal-image" anti-idiotypic monoclonal antibody (MAb) to elicit a rotavirus-specific antibody response. An immunoglobulin G2a MAb, designated RQ31 (MAb1), specific for the outer capsid protein VP4 of bovine Q17 rotavirus and capable of neutralizing viral infection in vitro was used to generate an anti-idiotypic MAb (MAb2). This MAb2, designated RQA2, was selected by enzyme-linked immunosorbent assay (ELISA) using F(ab')2 fragments of RQ31. RQA2 (MAb2) inhibited the binding of RQ31 (MAb1) to the virus but had no effect on the binding of other rotavirus-specific MAbs. The MAb2 also inhibited virus neutralization mediated by MAb1 in a dose-dependent fashion. Naive guinea pigs immunized with the MAb2 produced anti-anti-idiotypic antibodies (Ab3) that reacted with bovine Q17 rotavirus in an ELISA and neutralized rotavirus infection in vitro. The Ab3 response was characterized as MAb1-like because the Ab3 recognizes only the Q17 and neonatal calf diarrhea virus rotavirus strains in ELISA, as did RQ31 (MAb1). The Ab3 response also possessed two other characteristics of RQ31: the abilities to bind the 1.36 (double-capsid) but not the 1.38 (single-capsid) purified rotavirus fraction in ELISA and to immunoprecipitate the VP4 rotavirus protein. |
| Databáze: | OpenAIRE |
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