Differences in ethidium bromide and 4'-6-diamidino-2-phenylindole staining profiles with regard to DNA fragmentation during apoptosis
| Autor: | Susumu Ito, Ichiro Shimizu, Aya Hiasa, Hirohito Honda, Masayuki Shono, Yumiko Tomita, Toshihiro Omoya |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Indoles Clinical Biochemistry Apoptosis DNA Fragmentation Biology Biochemistry Stain Antibodies Mice chemistry.chemical_compound Ethidium Genetics Fluorescence microscope Animals fas Receptor Molecular Biology Cells Cultured Fluorescent Dyes Cell Nucleus Mice Inbred BALB C Staining and Labeling Apoptotic DNA fragmentation DNA Cell Biology Molecular biology Intercalating Agents Staining Nuclear DNA Liver Microscopy Fluorescence chemistry Dactinomycin DNA fragmentation Ethidium bromide |
| Zdroj: | IUBMB Life. 46:1055-1061 |
| ISSN: | 1521-6543 |
| DOI: | 10.1080/15216549800204602 |
| Popis: | To simply and directly evaluate DNA fragmentation during apoptosis induced in mouse cultured hepatocytes by an anti-Fas antibody, we examined the fluorescence intensity in cell nuclei stained with ethidium bromide and 4'-6-diamidino-2-phenylindole by optiphoto fluorescence microscopy. The intensity of the former staining for the nuclear DNA of apoptotic cells was clearly decreased compared to that of non-apoptotic cells, whereas no difference in the fluorescence intensity for the latter stain between the apoptotic and non-apoptotic groups was observed. Thus, the use of optiphoto fluorescence microscopy, in conjunction with both stains, constitutes a useful tool for the evaluation of apoptotic DNA fragmentation. |
| Databáze: | OpenAIRE |
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