Thymol-Loaded Polymeric Nanoparticles Improve the Postharvest Microbiological Safety of Blueberries

Autor: Ab Rashid Syarifah, Woei-Yenn Tong, Chean-Ring Leong, Wen-Nee Tan, Chee-Keong Lee, Mohd Anuar Razealy, Hway Teo Siew, Siti Khalida Abdull Lazit, Jun-Wei Jun-Wei, Nur Amiera Syuhada Rozman
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Food Technology and Biotechnology
Volume 61
Issue 2
ISSN: 1334-2606
1330-9862
Popis: Research background. The presence of Yersinia enterocolitica on raw food products raises the concern of yersiniosis as most of the berries are consumed raw. This is a challenging issue from the food safety aspect since it could increase the occurrence of foodborne diseases among humans. Thus, it is crucial to implement an effective sanitation before the packaging. Experimental approach. This study aims to synthesize and characterize thymol-loaded polyvinyl alcohol (Thy/PVA) nanoparticles as a sanitizer for postharvest treatment of blueberries. Thy/PVA nanoparticles were characterized by spectroscopic and microscopic approaches, prior to the analyses of antimicrobial properties. Results and conclusions. The diameter size of the nanoparticles was on average 84.7 nm, with a surface charge of −11.73 mV. Based on Fourier transform infrared (FTIR) measurement, the Thy/PVA nanoparticles notably shifted to the frequency of 3275.70, 2869.66, 1651.02 and 1090.52 cm-1. A rapid burst was observed in the first hour of release study, and 74.9 % thymol was released from the PVA nanoparticles. The largest inhibition zone was displayed by methicillin-resistant Staphylococcus aureus (MRSA), followed by Y. enterocolitica and Salmonella typhi. However, amongst these bacteria, the inhibition and killing of Y. enterocolitica required a lower concentration of Thy/PVA nanoparticles. The treatment successfully reduced the bacterial load of Y. enterocolitica on blueberries by 100 %. Novelty and scientific contribution. Thymol is a plant-based chemical without reported adverse effects to humans. In this study, by using the nanotechnology method of encapsulation with PVA, we improved the stability and physicochemical properties of thymol. This nanoparticle-based sanitizer could potentially promote the postharvest microbiological safety of raw berries, which may become an alternative practice of food safety.
Pozadina istraživanja. Prisutnost bakterije Yersinia enterocolitica u sirovim prehrambenim proizvodima izaziva zabrinutost zbog moguće pojave jersinioze, s obzirom na to da se bobičasto voće najčešće konzumira sirovo. S aspekta sigurnosti hrane ova bakterija predstavlja problem, jer može povećati učestalost pojave bolesti koje se prenose hranom. Stoga je neophodno provesti učinkovitu sanitaciju voća prije pakiranja. Eksperimentalni pristup. Svrha je ovoga rada bila sintetizirati i okarakterizirati nanočestice poli(vinil-alkohola) s timolom kao dezinficijensom za obradu borovnica prije pakiranja. Nanočestice su okarakterizirane pomoću spektroskopije i mikroskopije, a zatim su ispitana njihova antimikrobna svojstva. Rezultati i zaključci. Prosječni promjer nanočestica bio je 84,7 nm, s površinskim nabojem od −11,73 mV. Rezultati Fourier transformirane infracrvene spektroskopije (FTIR) pokazuju značajan pomak pika nanočestica poli(vinil-alkohola) s timolom prema frekvencijama od 3275,70; 2869,66; 1651,02 i 1090,52 cm-1. Iznenadni porast količine timola oslobođenog iz nanočestica opažen je u prvih sat vremena ispitivanja njegovog kontroliranog otpuštanja, a ukupno je otpušteno 74,9 % timola. Opažena je najveća zona inihbicije rasta bakterije Staphylococcus aureus otporne na meticilin (MRSA), a zatim bakterija Y. enterocolitica i Salmonella typhi. Međutim, za inhibiciju i ubijanje bakterija Y. enterocolitica bile su potrebne manje koncentracije nanočestica poli(vinil-alkohola) s timolom. Ovim je postupkom broj bakterija Y. enterocolitica uspješno smanjen za 100 %. Novina i znanstveni doprinos. Timol je spoj biljnog porijekla, koji ne narušava zdravlje čovjeka. U ovome smo radu inkapsulacijom u omotaču s poli(vinil-alkoholom) povećali stabilnost i poboljšali fizikalno-kemijska svojstva timola. Primjenom nanočestica tog dezinficijensa može se poboljšati mikrobiološka ispravnost sirovog bobičastog voća, što se u praksi može primijeniti za očuvanje sigurnosti hrane.
Databáze: OpenAIRE
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