Pan-colonic pharmacokinetics of catechins and procyanidins in male Sprague–Dawley rats
| Autor: | Anne K. Ickes, Andrew P. Neilson, Katheryn M. Goodrich, Andrew T. Smithson |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
food.ingredient Colon Endocrinology Diabetes and Metabolism Metabolite Clinical Biochemistry Biological Availability Absorption (skin) Biochemistry Catechin Rats Sprague-Dawley chemistry.chemical_compound Cecum food Pharmacokinetics medicine Animals Proanthocyanidins Molecular Biology Nutrition and Dietetics Bacteria Grape Seed Extract Metabolism Rats Bioavailability medicine.anatomical_structure Epicatechin gallate chemistry Grape seed extract |
| Zdroj: | The Journal of Nutritional Biochemistry. 26:1007-1014 |
| ISSN: | 0955-2863 |
| DOI: | 10.1016/j.jnutbio.2015.04.008 |
| Popis: | Poor absorption and bioavailability of procyanidins from the upper gastrointestinal tract result in the majority of the dose reaching the colon. During colonic transit, progressive microbial metabolism likely produces gradients of procyanidins and microbial metabolites along the length of the colon, suggesting that proximal and distal regions are exposed to different profiles of procyanidins and metabolites. However, previous studies have largely treated the colon as a single organ or looked at fecal profiles, and differences in the profiles of native and metabolite compounds between regions have not been observed. The metabolism kinetics of procyanidins larger than trimers and formation of metabolites in the colon have not been well characterized. Therefore, the objective of this study was to determine the kinetics of delivery and microbial metabolism of monomeric, dimeric and oligomeric procyanidins in the cecum and proximal, mid and distal colon. Sprague–Dawley rats were gavaged grape seed extract and sacrificed over 18h. Analysis of luminal contents showed distinct native and metabolite profiles for each region. Procyanidins had maximum concentrations at approximately 3h postgavage for all sections. Metabolites reached maximum concentrations from 3 to 18h postgavage. The appearance of metabolites was highly dependent on species: larger metabolites were found at earlier times in the more proximal segments, and smaller metabolites were found at later times in more distal regions. This study allowed for the observation of regions in the lower gastrointestinal tract, giving insight into the distribution and delivery of procyanidins and their microbial metabolites throughout the colon. |
| Databáze: | OpenAIRE |
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