Identification of key bacterial populations affecting early embryonic development in cattle uterus
| Autor: | Zhang Zijing, Xiangdong Yu, Zhaoxue Xu, Fuying Chen, Xin Xiaoling, Wang Eryao, Shi Qiaoting, Chenchen Lyu, Hua Liushuai, Bai Zhonglin |
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| Rok vydání: | 2020 |
| Předmět: |
animal structures
Animal breeding Firmicutes Uterus Embryonic Development Superovulation Biology Andrology Endometrium 03 medical and health sciences Pregnancy Proteobacteria medicine Animals 030304 developmental biology 0303 health sciences Embryogenesis 0402 animal and dairy science Bacteroidetes Embryo Sequence Analysis DNA 04 agricultural and veterinary sciences General Medicine Embryo Mammalian biology.organism_classification 040201 dairy & animal science medicine.anatomical_structure embryonic structures Cattle Female General Agricultural and Biological Sciences Embryo quality |
| Zdroj: | Animal Science Journal. 91 |
| ISSN: | 1740-0929 1344-3941 |
| Popis: | Superovulation is an important animal breeding biotechnology, while the quality of embryos obtained from superovulation is unstable in cattle. The relationship between the microorganisms in the cattle uterus and embryo qualities was determined to identify the key bacterial populations affecting early embryonic development. A total of 10 Xia Nan cows underwent superovulation, we collected cervical mucus and flush samples to investigated by 16S rDNA sequencing. Results showed that there were abundant microorganisms in cervical mucus, but no obvious relationship with the quality of embryos. The clustering results of flush samples were consistent with the grouping of embryo quality. Proteobacteria accounted for more than 95% of the total bacterial community in group A with the best embryo quality (qualified embryo ratio above 0.8), and as embryo quality decreased, the Proteobacteria proportion also decreased. In contrast to the proportion of Proteobacteria, the proportions of Firmicutes and Bacteroidetes significantly increased as embryo quality decreased. For group C with the worst embryo quality, the proportions of Firmicutes and Bacteroidetes increased to 4.7 times and 12.3 times of group A, respectively. These results showed that the quantities and proportions of Firmicutes and Bacteroidetes may be related to early embryonic development in cattle. |
| Databáze: | OpenAIRE |
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