CircRNA-1926 Promotes the Differentiation of Goat SHF Stem Cells into Hair Follicle Lineage by miR-148a/b-3p/CDK19 Axis
| Autor: | Man Bai, Ze Y Wang, Wen L. Bai, Yu B. Zhu, Rong H. Yin, Qian Jiao, Su J. Zhao, Yi X Fan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Biology
Keratin 17 Article Circular RNA lcsh:Zoology microRNA medicine lcsh:QL1-991 Gene post-transcriptional lcsh:Veterinary medicine General Veterinary integumentary system bulge RNA circular RNA Hair follicle cashmere Cell biology induced differentiation medicine.anatomical_structure Keratin 7 lcsh:SF600-1100 Animal Science and Zoology methylation Stem cell |
| Zdroj: | Animals : an Open Access Journal from MDPI Animals, Vol 10, Iss 1552, p 1552 (2020) Animals Volume 10 Issue 9 |
| ISSN: | 2076-2615 |
| Popis: | Circular RNAs (CircRNAs) are a type of non-coding RNAs, which contain a covalently closed loop structure without 5&prime to 3&prime free ends. CircRNAs play essential roles in the regeneration of secondary hair follicle (SHF) and cashmere growth in goats. CircRNA-1926 was previously identified in SHF of cashmere goats, but its potential roles are unclear. In this study, we confirmed the expression of circRNA-1926 in SHF bulge of nine cashmere goats with a significantly higher level at anagen than that of telogen. Through the use of both overexpression and siRNA interference, we showed that circRNA-1926 promoted the differentiation of SHF stem cell into hair follicle lineage in cashmere goats which was evaluated via indictor genes Keratin 7 and Keratin 17. Using RNA pull-down, we found that circRNA-1926 bound with miR-148a/b-3p. Additionally, our data indicated that circRNA-1926 promoted the expression of the CDK19 gene. Using dual-luciferase reporter assays, it was revealed that circRNA-1926 positively regulated the CDK19 expression through miR-148a/b-3p. The results from this study demonstrated that circRNA-1926 contributes the differentiation of SHF stem cells into hair follicle lineages in cashmere goats via sponging miR-148a/b-3p to enhance CDK19 expression. |
| Databáze: | OpenAIRE |
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