Microbial production of dihomo-γ-linolenic acid by Δ5-desaturase gene-disruptants of Mortierella alpina 1S-4
| Autor: | Hiroshi Kikukawa, Eiji Sakuradani, Jun Ogawa, Sakayu Shimizu, Akinori Ando, Tomoyo Okuda |
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| Rok vydání: | 2016 |
| Předmět: |
Fatty Acid Desaturases
0301 basic medicine DNA End-Joining Repair DNA Ligases Auxotrophy 030106 microbiology Bioengineering Biology Applied Microbiology and Biotechnology Metabolic engineering 03 medical and health sciences chemistry.chemical_compound 8 11 14-Eicosatrienoic Acid Bioreactors Delta-5 Fatty Acid Desaturase Mortierella Gene chemistry.chemical_classification DNA ligase Arachidonic Acid Dihomo-γ-linolenic acid Uracil biology.organism_classification 030104 developmental biology Metabolic Engineering chemistry Biochemistry Gene Targeting Arachidonic acid Biotechnology |
| Zdroj: | Journal of Bioscience and Bioengineering. 122:22-26 |
| ISSN: | 1389-1723 |
| DOI: | 10.1016/j.jbiosc.2015.12.007 |
| Popis: | We constructed dihomo-γ-linolenic acid (DGLA)-producing strains with disruption of the Δ5-desaturase (Δ5ds) gene, which encodes a key enzyme catalyzing the bioconversion of DGLA to arachidonic acid (ARA), by efficient gene-targeting, using Δlig4 strain of Mortierella alpina 1S-4 as the host. In previous study, we had already identified and disrupted the lig4 gene encoding DNA ligase 4, which involves in non-homologous end joining, in M. alpina 1S-4, and the Δlig4 strain had showed efficient gene-targeting. In this study, the uracil auxotroph of Δlig4 strain was constructed, and then transformed for disruption of Δ5ds. The isolation of nine Δ5ds-disruptants out of 18 isolates indicated that the disruption efficiency was 50%. The ratio of DGLA among the total fatty acids of the Δ5ds-disruptants reached 40.1%; however, no ARA was detected. To our knowledge, this is the first study to report the construction of DGLA-producing transformants by using the efficient gene-targeting system in M. alpina 1S-4. |
| Databáze: | OpenAIRE |
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