Label-free, high-throughput electrical detection of cells in droplets
| Autor: | Loes I. Segerink, Albert van den Berg, I. Vermes, Evelien W. M. Kemna, Floor Wolbers |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
IR-86821
endocrine system Materials science Cell Survival Microfluidics METIS-297030 Nanotechnology 02 engineering and technology 01 natural sciences Biochemistry complex mixtures Buffer (optical fiber) Analytical Chemistry law.invention Mice law Cell Line Tumor Electrochemistry Environmental Chemistry Animals Electrical impedance Spectroscopy Resistive touchscreen business.industry 010401 analytical chemistry technology industry and agriculture Water Electrochemical Techniques Lab-on-a-chip Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology eye diseases 0104 chemical sciences High-Throughput Screening Assays Membrane Cytoplasm Electrode Optoelectronics 0210 nano-technology business |
| Zdroj: | The Analyst Analyst, 138(16), 4585-4592. Royal Society of Chemistry |
| ISSN: | 0003-2654 |
| DOI: | 10.1039/c3an00569k |
| Popis: | Today, droplet based microfluidics has become a standard platform for high-throughput single cell experimentation and analysis. However, until now no label-free, integrated single cell detection and discrimination method in droplets is available. We present here a microfluidic chip for fast (>100 Hz) and label-free electrical impedance based detection of cells in droplets. The microfluidic glass-PDMS device consists of two main components, the droplet generator and the impedance sensor. The planar electrode pair in the main channel allows the detection of only cells and cell containing droplets passing the electrodes using electrical impedance measurements. At a measurement frequency of 100 kHz non-viable cells, in low-conducting (LC) buffer, show an increase in impedance, due to the resistive effect of the membrane. The opposite effect, an impedance decrease, was observed when a viable cell passed the electrode pair, caused by the presence of the conducting cytoplasm. Moreover, we found that the presence of a viable cell in a droplet also decreased the measured electrical impedance. This impedance change was not visible when a droplet containing a non-viable cell or an empty droplet passed the electrode pair. A non-viable cell in a droplet and an empty droplet were equally classified. Hence, droplets containing (viable) cells can be discriminated from empty droplets. In conclusion, these results provide us with a valuable method to label-free detect and select viable cells in droplets. Furthermore, the proposed method provides the first step towards additional information regarding the encapsulated cells (e.g., size, number, morphology). Moreover, this all-electric approach allows for all-integrated Lab on a Chip (LOC) devices for cell applications using droplet-based platforms. |
| Databáze: | OpenAIRE |
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