Isolated perfused udder model for transcriptome analysis in response to Streptococcus agalactiae
| Autor: | Lyderson Facio Viccini, W. A. Carvalho, Danísio Prado Munari, Marcos Vinícius Gualberto Barbosa da Silva, Humberto M. Brandão, Marta Fonseca Martins, Ana Paula Sbardella, A.S. Guimarães, Mayara M. D. C. A. Weller, J. C. Gern, I. Fonseca, Maria Aparecida Vasconcelos Paiva Brito, Isabella S. B. Pinto |
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| Přispěvatelé: | Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Inst Fed Catarinense, Universidade Estadual Paulista (Unesp), Univ Fed Juiz de Fora, Universidade Federal do Espírito Santo (UFES) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Chemokine
CD14 medicine.disease_cause mastitis immune response Andrology Transcriptome 03 medical and health sciences Immune system Gene expression medicine Dairy cattle RNA-Seq 030304 developmental biology 0303 health sciences Innate immune system biology 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine mammary gland perfused 040201 dairy & animal science TLR2 Streptococcus agalactiae biology.protein Animal Science and Zoology Food Science |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | This study aimed to evaluate the transcriptional changes occurring in isolated perfused mammary alveolar tissue in response to inoculation withS. agalactiaeand to identify the most affected biological functions and pathways after 3 h. Four udders taken at slaughter from cows with healthy mammary gland were perfused ex situ with warmed and gassed Tyrode's solution. Mammary alveolar tissue samples were taken from the left fore and rear quarters (IQ-inoculated quarters) before inoculation (hour 0) and at 3 h post inoculation (hpi) and at the same times from control right fore and rear quarters (not inoculated: NIQ). A total of 1756 differentially expressed genes (DEGs) were identified between IQ and NIQ at 3 hpi using edgeR package. Within this set of DEGs, 952 were up regulated and mainly involved with innate immune response and inflammatory response, e.g.,CD14,CCL5,TLR2,IL-8,SAA3, as well as in transcriptional regulation such asFOS,STAT3andNFKBIA. Genes down-regulated (804) included those involved with lipid synthesis e.g.,APOC2,SCD,FABP3andFABP4. The most affected pathways were chemokine signaling, Wnt signaling and complement and coagulation cascades, which likely reflects the early stage response of mammary tissue toS. agalactiaeinfection. No significant gene expression changes were detected by RNA-Seq in the others contrasts. Real time-PCR confirmed the increase in mRNA abundance of immune-related genes:TLR2,TLR4,IL-1β, andIL-10at 3 hpi between IQ and NIQ. The expression profiles ofCasp1andBaxfor any contrasts were unaffected whereasBcl2was increased in IQ, which suggests no induction of apoptosis during the first hours after infection. Results provided novel information regarding the early functional pathways and gene network that orchestrate innate immune responses toS. agalactiaeinfection. This knowledge could contribute to new strategies to enhance resistance to this disease, such as genomic selection. |
| Databáze: | OpenAIRE |
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