High-content analysis of particulate matters-induced oxidative stress and organelle dysfunction in vitro
| Autor: | Guanglei Wang, Yufei Dai, Yong Niu, Jinling Gao, Shuguang Leng, Zhishang Chang, Xuxia Song, Xiaomei Zheng, Huawei Duan, Yuxin Zheng, Jinglong Tang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell Toxicology medicine.disease_cause Endoplasmic Reticulum Cell Line 03 medical and health sciences 0302 clinical medicine Organelle medicine Humans Fibroblast Vehicle Emissions Organelles Chemistry Endoplasmic reticulum Endothelial Cells Epithelial Cells General Medicine Fibroblasts Cell biology High-Throughput Screening Assays Mitochondria Oxidative Stress 030104 developmental biology medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis Toxicity Human umbilical vein endothelial cell Particulate Matter Lysosomes Oxidative stress |
| Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 59 |
| ISSN: | 1879-3177 |
| Popis: | Oxidative stress is usually considered to be a common mechanism by which particulate matter (PM) exposure induces adverse effects. However, the further biological events such as organelle dysfunction following oxidative stress remain to be explored. In this study, we applied high-content screening (HCS) technique to investigate the toxicological effects of carbon black (CB), diesel exhaust particle (DEP) and PM2.5 on oxidative stress and organelle function in human bronchial epithelial cell (16HBE), human embryo lung fibroblast cell (HELF) and human umbilical vein endothelial cell (HUVEC) which were used to represent distinct regions of the lung, and compared the toxicity impacts of different PMs and the sensitiveness of cell lines. We found three types of PMs induced mitochondrial dysfunction in three cell lines and lysosomal alkalinization in HUVEC while only CB triggered endoplasmic reticulum (ER) stress in 16HBE and HUVEC, and oxidative stress might mediate these processes. Moreover, CB basically exhibited more potent toxicity compared with DEP and PM2.5, which might be attributed to its less oxygen content. Finally, the finding that PMs-induced toxicity impacts exhibited a cell-type dependent manner might provide some information to help to understand the sensitivity of different tissue in the lung. |
| Databáze: | OpenAIRE |
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