Early embryonic cells from in vivo-produced goat embryos transmit the caprine arthritis–encephalitis virus (CAEV)
| Autor: | Yahia Chebloune, Gérard Chatagnon, A. Lamara, Claudie Fornazero, Laila Mselli-Lakhal, Daniel Tainturier, J.F. Bruyas, I. Battut, Francis Fieni |
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| Přispěvatelé: | Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Rok vydání: | 2002 |
| Předmět: |
Arthritis-Encephalitis Virus
Caprine animal structures 040301 veterinary sciences [SDV]Life Sciences [q-bio] Superovulation Biology Virus 0403 veterinary science Andrology Cytopathogenic Effect Viral Food Animals In vivo Culture Techniques medicine Animals Cloning Molecular Small Animals Zona pellucida Zona Pellucida Equine Goats 0402 animal and dairy science virus diseases Embryo 04 agricultural and veterinary sciences Embryo Mammalian 040201 dairy & animal science Embryonic stem cell Virology In vitro 3. Good health medicine.anatomical_structure embryonic structures Lentivirus Infections Oviduct Animal Science and Zoology Synovial membrane Estrus Synchronization |
| Zdroj: | Theriogenology Theriogenology, 2002, 58 (6), pp.1153-1163. ⟨10.1016/s0093-691x(02)00950-0⟩ |
| ISSN: | 0093-691X |
| DOI: | 10.1016/s0093-691x(02)00950-0 |
| Popis: | The aim of this study was to investigate whether cells of early goat embryos isolated from in vivo-fertilized goats interact with the caprine arthritis–encephalitis virus (CAEV) in vitro and whether the embryonic zona pellucida (ZP) protects early embryo cells from CAEV infection. ZP-free and ZP-intact 8–16 cell embryos were inoculated for 2 h with CAEV at the 10 4 tissue culture infectious dose 50 (TCID 50 )/ml. Infected embryos were incubated for 72 h over feeder monolayer containing caprine oviduct epithelial cells (COECs) and CAEV indicator goat synovial membrane (GSM) cells. Noninoculated ZP-free and ZP-intact embryos were submitted to similar treatments and used as controls. Six days postinoculation, infectious virus assay of the wash fluids of inoculated early goat embryos showed typical CAEV-induced cytopathic effects (CPE) on indicator GSM monolayers, with fluids of the first two washes only. The mixed cell monolayer (COEC+GSM) used as feeder cells for CAEV inoculated ZP-free embryos showed CPE. In contrast, none of the feeder monolayers, used for culture of CAEV inoculated ZP-intact embryos or the noninoculated controls, developed any CPE. CAEV exposure apparently did not interfere with development of ZP-free embryos in vitro during the 72 h study period when compared with untreated controls (34.6 and 36% blastocysts, respectively, P >0.05). From these results one can conclude that the transmission of infectious molecularly cloned CAEV–pBSCA (plasmid binding site CAEV) by embryonic cells from in vivo-produced embryos at the 8–16 cell stages is possible with ZP-free embryos. The absence of interactions between ZP-intact embryos and CAEV in vitro suggests that the ZP is an efficient protective embryo barrier. |
| Databáze: | OpenAIRE |
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