Nucleus pulposus phenotypic markers to determine stem cell differentiation : fact or fiction?
| Autor: | Thorpe Aa, Laura B. Creemers, Abbie L. A. Binch, Chris Sammon, Christine L. Le Maitre |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Cartilage
Articular Genetic Markers 0301 basic medicine Pathology medicine.medical_specialty nucleus pulposus phenotypic markers regeneration stem cells PAX1 FOXF1 Pathology Section Cellular differentiation Intervertebral Disc Degeneration Biology Research Support Mesenchymal Stem Cell Transplantation stem cells PAX1 03 medical and health sciences Chondrocytes Pathology Section medicine Journal Article Humans Paired Box Transcription Factors RNA Messenger Non-U.S. Gov't FOXF1 Intervertebral Disc Gene Keratin-19 Messenger RNA Guided Tissue Regeneration Cell adhesion molecule nucleus pulposus Regeneration (biology) Research Support Non-U.S. Gov't Mesenchymal stem cell Cell Differentiation Forkhead Transcription Factors Mesenchymal Stem Cells Hypoxia-Inducible Factor 1 alpha Subunit phenotypic markers Phenotype Research Paper: Pathology Cell biology 030104 developmental biology Oncology Genetic marker regeneration Cell Adhesion Molecules |
| Zdroj: | Oncotarget, 7(3), 2189. Impact Journals Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | Progress in mesenchymal stem cell (MSC) based therapies for nucleus pulposus (NP) regeneration are hampered by a lack of understanding and consensus of the normal NP cell phenotype. Despite the recent consensus paper on NP markers, there is still a need to further validate proposed markers. This study aimed to determine whether an NP phenotypic profile could be identified within a large population of mature NP samples.\ud \ud qRT-PCR was conducted to assess mRNA expression of 13 genes within human non-degenerate articular chondrocytes (AC) (n=10) and NP cells extracted from patients across a spectrum of histological degeneration grades (n=71). qRT-PCR results were used to select NP marker candidates for protein expression analysis.\ud \ud Differential expression at mRNA between AC and non-degenerate NP cells was only observed for Paired Box Protein 1 (PAX1) and Forkhead box F1 (FOXF1). In contrast no other previously suggested markers displayed differential expression between non-degenerate NP and AC at mRNA level. PAX1 and FOXF1 protein expression was significantly higher in the NP compared to annulus fibrosus (AF), cartilaginous endplate (CEP) and AC. In contrast Laminin-5 (LAM-332), Keratin-19 (KRT-19) and Hypoxia Inducible Factor 1 alpha (HIF1α) showed no differential expression in NP cells compared with AC cells.\ud \ud A marker which exclusively differentiates NP cells from AF and AC cells remains to be identified, raising the question: is the NP a heterogeneous population of cells? Or does the natural biological variation during IVD development, degeneration state and even the life cycle of cells make finding one definitive marker impossible? |
| Databáze: | OpenAIRE |
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