Droplet digital PCR as a sensitive tool to assess exposure pressure from Echinococcus multilocularis in intermediate hosts
| Autor: | Kensuke Mori, Alessandro Massolo, Colin Nicholas, Gérald Umhang, Amanda Gerber, Stefano Liccioli, Claudia Klein |
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| Přispěvatelé: | University of Pisa - Università di Pisa, University of Calgary, Laboratoire de la rage et de la faune sauvage de Nancy (LRFSN), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Institute of Farm Animal Genetics (ING), Friedrich-Loeffler-Institut (FLI), This work was supported by the Faculty of Veterinary Medicine (to AM) and by the Park sector of the City of Calgary (to AM). |
| Rok vydání: | 2021 |
| Předmět: |
Prevalence estimates
Rodent [SDV]Life Sciences [q-bio] Veterinary (miscellaneous) 030231 tropical medicine Prevalence ddPCR Rodentia Biology Real-Time Polymerase Chain Reaction Echinococcus multilocularis Exposure pressure Real-time PCR Rodents Sensitivity Animals Echinococcosis 030308 mycology & parasitology Lesion 03 medical and health sciences 0302 clinical medicine biology.animal medicine Parasite hosting [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology Digital polymerase chain reaction 0303 health sciences Intermediate host biology.organism_classification Virology 3. Good health Infectious Diseases Real-time polymerase chain reaction Insect Science Parasitology medicine.symptom |
| Zdroj: | Acta Tropica Acta Tropica, Elsevier, 2021, 223, pp.106078. ⟨10.1016/j.actatropica.2021.106078⟩ |
| ISSN: | 0001-706X |
| DOI: | 10.1016/j.actatropica.2021.106078 |
| Popis: | International audience; A key element to understanding parasite epidemiology is assessing their prevalence in the respective wild reservoir hosts. The tapeworm Echinococcus multilocularis circulates between canid species (definite hosts) and small mammals (mostly rodents; intermediate hosts). Prevalence rates of Echinococcus multilocularis in the intermediate host are most exclusively determined through macroscopic examination of the liver generally followed by molecular or histological diagnostic for parasite species confirmation. The overall objective of the study was to investigate the suitability of Real-Time PCR and Droplet Digital PCR (ddPCR) analysis as tool to detect exposure pressure (frequency of infection events) from E. multilocularis in intermediate hosts even in the absence of macroscopic lesions in the liver. One hundred six small mammals (meadow voles and deer mice) were trapped followed by post-mortem examination including macroscopic evaluation of the liver to detect lesions indicative of infection with Echinococcus multilocularis but also by sampling a piece of liver in absence of lesion to submit it to molecular assay. Macroscopic lesions were present in the livers of two samples. Including the latter two samples, five samples yielded a positive result following Real-Time PCR, whereas 16 samples displayed three or more positive droplets upon ddPCR and were considered positive. Whether these additional cases without macroscopic lesions would have become infectious during the lifespan of the rodent or were abortive or early infections is unclear, but these data suggest levels of exposure of intermediate hosts to the parasite is much higher than assumed. |
| Databáze: | OpenAIRE |
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