Evaluation the cytotoxic effect of cytotoxin-producing Klebsiella oxytoca isolates on the HEp-2 cell line by MTT assay
| Autor: | Leila Lormohammadi, Mohammad Mehdi Soltan-Dallal, Jalil Fallah-Mehrabadi, Masoumeh Douraghi, Majid Validi |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Serial dilution Cell Survival Tetrazolium Salts Iran Urine Microbiology Stain Klebsiella spp Feces 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Humans Cytotoxic T cell MTT assay 030212 general & internal medicine Formazans biology Cytotoxins Klebsiella oxytoca Sputum biology.organism_classification Klebsiella Infections Blood 030104 developmental biology Infectious Diseases Hep 2 cell Wounds and Injuries |
| Zdroj: | Microbial Pathogenesis. 113:416-420 |
| ISSN: | 0882-4010 |
| Popis: | Background The cytotoxic effects on epithelial cells of the human are not observed in other strains of Klebsiella spp and are only observed in K. oxytoca strains. MTT assay was used to evaluate cytotoxic activity. In this study, colorimetric method was used to evaluate the cytotoxic effect of cytotoxin-producing isolates on Hep-2 cell line and determines the percentage of surviving cells. Materials and methods In this study, we collected a total of 75 K. oxytoca strains isolate and we detected the production of toxins and their cytotoxic effects on HEp-2 cells. Colorimetric method such as MTT assay was used to evaluate the cytotoxic effect of cytotoxin-producing isolates on Hep-2 cell line and determines the percentage of surviving cells. Results Nine isolates had cytotoxic effects on HEp-2 cells. The results of MTT assay showed that the isolated strains were different from the control stain in terms of toxinogenicity and cytotoxic effects on HEp-2 cells at the studied dilutions (1:3, 1:6, 1:12, 1:24, 1:48, and 1:96). Conclusions In the current study, Percentage of Hep-2 surviving cells exposed to 1:3, 1:6, 1:12, 1:24, 1:48, and 1:96 supernatant dilutions of cytotoxin-producing Klebsiella oxytoca isolates was different. |
| Databáze: | OpenAIRE |
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