In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens
| Autor: | Francine Carla Cadoná, Dbr Leal, Ibm Cruz, Cep Zimmermann, Jas Jaques, Alencar Kolinski Machado, Régis Adriel Zanette, Claudia Lautert, Karine Bizzi Schlemmer, Jânio Morais Santúrio |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species Aflatoxin Aflatoxin B1 lcsh:Veterinary medicine Broilers In vitro cytotoxicity Broiler Biology Molecular biology Peripheral blood mononuclear cell Oxidative damage chemistry Biochemistry lcsh:Zoology PicoGreen lcsh:SF600-1100 Cytotoxic T cell Animal Science and Zoology Lymphocytes lcsh:Animal culture lcsh:QL1-991 Viability assay lcsh:SF1-1100 |
| Zdroj: | Brazilian Journal of Poultry Science, Vol 16, Iss 3, Pp 307-312 (2014) |
| ISSN: | 1516-635X |
| DOI: | 10.1590/1516-635x1603307-312 |
| Popis: | The aim of the present work was to study the in-vitro cytotoxic effects of different concentrations of aflatoxin B1 (AFB1) on broiler lymphocytes. Lymphocyte-rich mononuclear cells were separated by Ficoll-Histopaque density and cultured in 96-wellplates containing the evaluated AFB1 concentrations in 5% CO2 atmosphere at 39°C. Thereafter, MTT, PicoGreen, and reactive oxygen species assays were performed. Cell viability decreased in the presence of 10 µg/mL AFB1 at 48 h (p < 0.05) and of 10 and 20 µg/mL AFB1 at 72 h (p < 0.01 and p < 0.001, respectively) when compared to the control (0 µg/mL). However, a dose-dependent increase in the cell-free DNA at 24 h was observed at 1, 10 and 20 µg/mL (p < 0.001). ROS formation significantly increased at 24 h at all concentrations (p < 0.001). The in-vitro results demonstrate that AFB1 is cytotoxic and causes biomolecular oxidative damage in broiler lymphocytes. |
| Databáze: | OpenAIRE |
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