Quantification of Epstein–Barr virus DNA load in nasopharyngeal brushing samples in the diagnosis of nasopharyngeal carcinoma in southern China
Autor: | Xiao Hui Zheng, Li Xia Lu, Xi Zhao Li, Wei Hua Jia |
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Rok vydání: | 2015 |
Předmět: |
Adult
Male Immunoglobulin A China Epstein-Barr Virus Infections Herpesvirus 4 Human Cancer Research Adolescent Nasopharyngeal neoplasm Antibodies Viral Virus disease progress Young Adult nasopharyngeal brush Diagnosis medicine Humans Antigens Viral Epstein–Barr virus infection Aged Nasopharyngeal Carcinoma biology Carcinoma Antibody titer Nasopharyngeal Neoplasms Original Articles General Medicine Middle Aged Viral Load medicine.disease Virology Real-time polymerase chain reaction Oncology Nasopharyngeal carcinoma Epstein–Barr virus DNA load DNA Viral Immunology biology.protein Capsid Proteins Female Viral load |
Zdroj: | Cancer Science |
ISSN: | 1349-7006 1347-9032 |
Popis: | Nasopharyngeal carcinoma (NPC) is highly incident in southern China, where 40% of world's new cases arise each year. Detection of Epstein–Barr virus (EBV) DNA load in nasopharyngeal (NP) brush/swab samples has gradually been established as a method for diagnosis of NPC. However, its applicable value in NPC diagnosis has never been investigated in southern China. It is important to explore whether such a test could be applicable to our local population. A total of 245 consecutive participants undergoing NP brushing examination were recruited to obtain the NP brushing samples in this study. Quantitative PCR assays were used to obtain the EBV DNA load. Mann–Whitney, ANOVA and receiver operating characteristic tests were used to analyze its diagnostic value. NP brushing samples from NPC patients showed extremely high levels of EBV DNA load (mean = 46360 copy/ng DNA) compared to its expression from non-NPC control (mean = 28 copy/ng DNA) and high-risk control (mean = 50 copy/ng DNA) groups. It produced 96% sensitivity and 97% specificity, at the COV = 225 copy/ng DNA. Furthermore, EBV DNA load could reflect disease progress. Our data showed a better performance of EBV DNA load in NP brushing samples compared with an initial biopsy, immunoglobulin A (IgA) antibody titers to viral capsid antigen in serum and EBV DNA load in plasma. Detection of EBV DNA load in NP brushing samples could be an effective supplement for NPC diagnosis. Being minimally invasive and low cost, NP brush sampling combined with EBV DNA detection demonstrates great potential for screening high-risk populations for NPC. |
Databáze: | OpenAIRE |
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