Delivery of Bioactive Gene Particles via Gelatin-Collagen-PEG-Based Electrospun Matrices
| Autor: | Eleni K. Tsekoura, Porntipa Pankongadisak, Daniel Graf, Hasan Uludağ, Teo Atz Dick, Yaman Boluk |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
collagen
food.ingredient Pharmaceutical Science 02 engineering and technology Polyethylene glycol electrospun matrices Gene delivery Gelatin Article Green fluorescent protein gelatin 03 medical and health sciences chemistry.chemical_compound Pharmacy and materia medica food plasmid DNA Tissue engineering Drug Discovery PEG ratio gene delivery 030304 developmental biology 0303 health sciences Polyethylenimine Bone Morphogenetic Protein technology industry and agriculture 021001 nanoscience & nanotechnology Electrospinning RS1-441 chemistry Biophysics Medicine Molecular Medicine 0210 nano-technology |
| Zdroj: | Pharmaceuticals Volume 14 Issue 7 Pharmaceuticals, Vol 14, Iss 666, p 666 (2021) |
| ISSN: | 1424-8247 |
| Popis: | The fabrication of fiber mats via electrospinning has been adopted in the last decades to produce high quality scaffolds for tissue engineering. However, an effective combination of electrospinning methods with gene delivery therapies remains a challenge. In this study, we describe how the delivery of gene complexes via electrospun mats that contain different volumes of gelatin (Gel), collagen (Col), and polyethylene glycol (PEG) can affect gene expression by transfected cells. Non-viral complexes were formulated by using lipid modified polyethylenimine (PEI) polymer and plasmid DNAs (pDNA) like the reporter Green Fluorescent Protein (GFP) and the therapeutically relevant Bone Morphogenetic Protein-2 (BMP-2) and electrospuned after being mixed with different volumes of Gel-Col-PEG mats and delivered to human myoblast (C2C12) and mouse osteoblast cells (MC3T3). The entrapment of GFP complexes via different homogeneous electrospun fiber mats revealed that a high fraction of collagen in the mats affected the quality of the fibers and led to reduced transfection efficiency on target cells. On the other hand, the fabrication of double-layered mats that contained collagen without complexes as a first layer and gelatin-collagen-PEG with complexes as a second layer successfully induced GFP expression and ALP activity in C2C12 cells. We conclude that this study has established the advantage of formulating multilayered bioactive collagen-based mats for gene delivery applications. |
| Databáze: | OpenAIRE |
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