AEG-1 aggravates inflammation via promoting NALP3 inflammasome formation in murine endometriosis lesions

Autor: Yahong Tong, Menghua Wang, Huan Xiao, Weizhi Chen, Juan Zhao, Jie Gao, Yanrong Jin, Qin Zhou, Wei Ma, Chunling Li, Xiuling Zhao
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Animal Cells and Systems, Vol 23, Iss 6, Pp 407-413 (2019)
Animal Cells and Systems
ISSN: 2151-2485
1976-8354
Popis: Endometriosis (EMs) is one of the most common gynaecological diseases in women of childbearing age. Astrocyte elevated gene-1 (AEG-1) is associated with the invasion, migration, apoptosis and prognosis of various cancers. However, the roles of AEG-1 in EMs and its corresponding molecular mechanism are still unknown. In this study, animal models of EMs were established and mice were divided into two groups (n = 10): Sham group and EMs group. The EMs cells were isolated from EMs model. The AEG-1 gene was knocked down by shRNA, while the SOCS1 gene was knocked down by siRNA. Histological changes, AEG-1 expression in tissues and inflammatory factors level were detected by H&E staining, immunohistochemistry and ELISA, respectively. RT-qPCR and western blotting were used to determine the expression level of related proteins. The present study found AEG-1 was up-regulated in the EMs model. Enhanced AEG-1 promoted inflammatory cell infiltration, and elevated the levels of IL-1β, IL-6, and TNF-α in EM group (p
Databáze: OpenAIRE
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