ROLE OF REACTIVE OXYGEN SPECIES DURING HYPERTENSION IN RESPONSE TO CHRONIC ANTI-ANGIOGENIC FACTOR (sFlt-1) EXCESS IN PREGNANT RATS
| Autor: | Babbette LaMarca, Lillian Fournier, Sydney R. Murphy, James N. Martin, Kathy Cockrell, Joey P. Granger, Marietta Arany, Kiran Tam Tam |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty Mean arterial pressure Blood Pressure medicine.disease_cause Article Preeclampsia Rats Sprague-Dawley chemistry.chemical_compound Pregnancy Internal medicine Internal Medicine medicine Animals reproductive and urinary physiology chemistry.chemical_classification Reactive oxygen species Vascular Endothelial Growth Factor Receptor-1 business.industry Hypertension Pregnancy-Induced medicine.disease Rats Blood pressure Endocrinology chemistry embryonic structures Gestation Female business Reactive Oxygen Species Nicotinamide adenine dinucleotide phosphate Oxidative stress Soluble fms-like tyrosine kinase-1 |
| Popis: | BACKGROUND Preeclampsia is associated with increased levels of reactive oxygen species (ROS) and the antiangiogenic factor, soluble fms-like tyrosine kinase-1 (sFlt-1). Moreover, recent studies have indicated that chronic sFlt-1 excess causes hypertension in pregnant animals. The purpose of this study was to evaluate the role of ROS in mediating sFlt-1-induced hypertension in the pregnant rat. METHODS Mean arterial pressure (MAP), and plasma sFlt-1 and tissue ROS levels were measured in the following groups: (i) pregnant controls; (ii) sFlt-1-treated pregnant rats; (iii) Tempol-treated pregnant rats; (iv) sFlt-1- and Tempol-treated pregnant rats. RESULTS MAP increased from 104 ± 2 mm Hg in pregnant control rats to 118 ± 3 mm Hg (P = 0.002) in sFlt-1-infused rats. Basal and nicotinamide adenine dinucleotide phosphate (NADPH)-stimulated levels of tissue ROS were increased in response to excess sFlt-1 during pregnancy. Pretreatment with Tempol attenuated oxidative stress and hypertension in response to sFlt-1. CONCLUSIONS ROS play an important role in mediating hypertension in response to chronic sFlt-1 excess during pregnancy. |
| Databáze: | OpenAIRE |
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