A Unique Arabinose 5-Phosphate Isomerase Found within a Genomic Island Associated with the Uropathogenicity of Escherichia coli CFT073
| Autor: | Timothy C. Meredith, Pan-Fen Wang, Alejandra Yep, Sara N. Smith, Ronald W. Woodard, Joshua A. Mosberg, Harry L. T. Mobley, Tod P. Holler |
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| Rok vydání: | 2011 |
| Předmět: |
Arabinose
Isomerase activity Genomic Islands Operon Molecular Sequence Data Isomerase medicine.disease_cause Microbiology Gene Expression Regulation Enzymologic Mice chemistry.chemical_compound Genomic island Cystitis Escherichia coli medicine Animals Uropathogenic Escherichia coli Amino Acid Sequence Molecular Biology Aldose-Ketose Isomerases Escherichia coli Infections Shigella boydii biology Escherichia coli Proteins Gene Expression Regulation Bacterial biology.organism_classification Enzymes and Proteins Enterobacteriaceae chemistry Biochemistry Mutation Kidney Diseases |
| Zdroj: | Journal of Bacteriology. 193:2981-2988 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.00033-11 |
| Popis: | Previous studies showed that deletion of genes c3405 to c3410 from PAI- metV , a genomic island from Escherichia coli CFT073, results in a strain that fails to compete with wild-type CFT073 after a transurethral cochallenge in mice and is deficient in the ability to independently colonize the mouse kidney. Our analysis of c3405 to c3410 suggests that these genes constitute an operon with a role in the internalization and utilization of an unknown carbohydrate. This operon is not found in E. coli K-12 but is present in a small number of pathogenic E. coli and Shigella boydii strains. One of the genes, c3406, encodes a protein with significant homology to the sugar isomerase domain of arabinose 5-phosphate isomerases but lacking the tandem cystathionine beta-synthase domains found in the other arabinose 5-phosphate isomerases of E. coli . We prepared recombinant c3406 protein, found it to possess arabinose 5-phosphate isomerase activity, and characterized this activity in detail. We also constructed a c3406 deletion mutant of E. coli CFT073 and demonstrated that this deletion mutant was still able to compete with wild-type CFT073 in a transurethral cochallenge in mice and could colonize the mouse kidney. These results demonstrate that the presence of c3406 is not essential for a pathogenic phenotype. |
| Databáze: | OpenAIRE |
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