Antagonists of GPR35 Display High Species Ortholog Selectivity and Varying Modes of Action
| Autor: | Graeme Milligan, Debra L. Taylor, Edward G. McIver, Craig Southern, Zaynab Neetoo-Isseljee, Laura Jenkins, Amanda E. Mackenzie, Jennifer E. Lappin, Nicholas Harries, Stuart A. Nicklin |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Cellular and Molecular
Bioluminescence Resonance Energy Transfer Techniques Agonist Arrestins G protein medicine.drug_class Cell Culture Techniques Pharmacology Biology Ligands Transfection Receptors G-Protein-Coupled Mice chemistry.chemical_compound Species Specificity medicine Animals Humans Potency Binding site Mode of action Receptor beta-Arrestins Dose-Response Relationship Drug Molecular Structure Hydrazones Thiourea beta-Arrestin 2 Rats Drug Partial Agonism Aminosalicylic Acids HEK293 Cells Microscopy Fluorescence Biochemistry chemistry Thiazolidines Molecular Medicine Zaprinast GPR35 Protein Binding |
| Zdroj: | Journal of Pharmacology and Experimental Therapeutics. 343:683-695 |
| ISSN: | 1521-0103 0022-3565 |
| DOI: | 10.1124/jpet.112.198945 |
| Popis: | Variation in pharmacology and function of ligands at species orthologs can be a confounding feature in understanding the biology and role of poorly characterized receptors. Substantial selectivity in potency of a number of GPR35 agonists has previously been demonstrated between human and rat orthologs of this G protein-coupled receptor. Via a bioluminescence resonance energy transfer-based assay of induced interactions between GPR35 and β-arrestin-2, addition of the mouse ortholog to such studies indicated that, as for the rat ortholog, murine GPR35 displayed very low potency for pamoate, whereas potency for the reference GPR35 agonist zaprinast was intermediate between the rat and human orthologs. This pattern was replicated in receptor internalization and G protein activation assays. The effectiveness and mode of action of two recently reported GPR35 antagonists, methyl-5-[(tert-butylcarbamothioylhydrazinylidene)methyl]-1-(2,4-difluorophenyl)pyrazole-4-carboxylate (CID-2745687) and 2-hydroxy-4-[4-(5Z)-5-[(E)-2-methyl-3-phenylprop-2-enylidene]-4-oxo-2-sulfanylidene-1,3-thiazolidin-3-yl]butanoylamino)benzoic acid (ML-145), were investigated. Both CID-2745687 and ML-145 competitively inhibited the effects at human GPR35 of cromolyn disodium and zaprinast, two agonists that share an overlapping binding site. By contrast, although ML-145 also competitively antagonized the effects of pamoate, CID-2745687 acted in a noncompetitive fashion. Neither ML-145 nor CID-2745687 was able to effectively antagonize the agonist effects of either zaprinast or cromolyn disodium at either rodent ortholog of GPR35. These studies demonstrate that marked species selectivity of ligands at GPR35 is not restricted to agonists and considerable care is required to select appropriate ligands to explore the function of GPR35 in nonhuman cells and tissues. |
| Databáze: | OpenAIRE |
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