Construction of dye-stapled Quenchbodies by photochemical crosslinking to antibody nucleotide-binding sites

Autor:	Kenji Matsumoto, Shuya Itayama, Mitsuru Shindo, Hiroshi Ueda, Jinhua Dong, Ryoji Abe, Hee-Jin Jeong, Kozue Kodama
Rok vydání:	2017
Předmět:	Indoles Ultraviolet Rays 02 engineering and technology 010402 general chemistry Photochemistry 01 natural sciences Antibodies Catalysis Materials Chemistry Nucleotide Nucleotide binding sites Binding site Fluorescent Dyes chemistry.chemical_classification Binding Sites biology Nucleotides Chemistry Metals and Alloys General Chemistry Photochemical Processes 021001 nanoscience & nanotechnology Cross-Linking Reagents Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Ceramics and Composites biology.protein Antibody 0210 nano-technology
Zdroj:	Chemical Communications. 53:10200-10203
ISSN:	1364-548X 1359-7345
DOI:	10.1039/c7cc03043f
Popis:	We successfully converted an antibody single-chain variable fragment and a full-sized antibody to Quenchbodies, which are a type of powerful fluorescent immunosensor, through ultraviolet-based photochemical crosslinking of an indole-3-butyric acid-conjugated fluorescent dye to the nucleotide-binding sites near the antigen-binding sites.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af96647614220fea293941d46270aee9 https://doi.org/10.1039/c7cc03043f Zobrazit plný text záznamu