Elevated levels of α-dicarbonyl compounds in the plasma of type II diabetics and their relevance with diabetic nephropathy
| Autor: | Zhuo-Feng Liu, Xin-Jie Wang, Wen-Yun Gao, Sheng-Bo Ma, Heng Li |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Clinical Biochemistry Diacetyl Deoxyglucose 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Gastroenterology Analytical Chemistry Diabetic nephropathy 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Internal medicine Diabetes mellitus Blood plasma medicine Humans Diabetic Nephropathies Chromatography High Pressure Liquid Aged Chromatography 010401 analytical chemistry Methylglyoxal Type 2 Diabetes Mellitus Glyoxal Cell Biology General Medicine Middle Aged Pyruvaldehyde medicine.disease 0104 chemical sciences Diabetes Mellitus Type 2 chemistry Female Quantitative analysis (chemistry) Biomarkers |
| Zdroj: | Journal of Chromatography B. :19-25 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2018.12.027 |
| Popis: | The presence of α‑dicarbonyl compounds (α-DCs) in vivo has been associated with the development of complications of diabetes mellitus (DM) and also with other chronic diseases. Therefore, quantitative analysis of α-DCs in body fluids is crucial to understand their roles in the formation of these chronic diseases. We established in this study a practical HPLC-UV method to measure 3‑deoxyglucosone (3-DG), glyoxal (GO), methylglyoxal (MGO), diacetyl (DA), and pentane‑2,3‑dione (PD) in blood plasma using 4‑(2,3‑dimethyl‑6‑quinoxalinyl)‑1,2‑benzenediamine (DQB) as a derivatizing reagent. The derivatizing reaction could be carried out quickly under mild conditions and the HPLC determination is simple, sensitive, and easy to operate. The recoveries of the α-DCs are between 85.26% and 110.20% (intra-day) and 87.25% and 103.18% (inter-day); the RSDs are between 1.28% and 5.69% (intra-day) and 2.26% and 6.34% (inter-day). We found the plasma levels of 3-DG, GO, and MGO in the diabetic patients are all significantly higher than those in healthy subjects. The results also show that the contents of GO and MGO in diabetic nephropathy (DN) patients are both significantly higher than those in simple T2DM patients. Moreover, it is found for the first time that the plasma level of GO might be a potential predictor of DN. The developed method would be useful for the measurements of the plasma α-DCs and the data acquired could be informative in the diagnosis of DM complications. |
| Databáze: | OpenAIRE |
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