Regulation of tissue-specific expression of the esterase S gene inDrosophila virilis
| Autor: | Grigorii N. Yenikolopov, Natalia Peunova, B. A. Kuzin, Georgiev Gp, Ruben A. Khechumian, Leonid I. Korochkin, Pavel V. Sergeev |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Male Untranslated region Transcription Genetic Molecular Sequence Data Biology Transfection Carboxylesterase Cell Line Gene product Open Reading Frames Genetics Animals Drosophila Proteins Point Mutation Tissue Distribution Amino Acid Sequence RNA Messenger ORFS Codon Promoter Regions Genetic Gene Regulation of gene expression Base Sequence Promoter biology.organism_classification Molecular biology Drosophila virilis Open reading frame Gene Expression Regulation Drosophila Female Carboxylic Ester Hydrolases Chickens |
| Zdroj: | Nucleic Acids Research. 21:3545-3551 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/21.15.3545 |
| Popis: | The esterase S gene (estS) of Drosophila virilis is specifically expressed in the ejaculatory bulbs of males. Its sequencing shows similarities between estS product and other esterases of different origin. The transcription of estS in ejaculatory bulbs is at least 2 orders of magnitude higher than in other tissues of males. Two promoters, P1 (distal) and P2 (proximal), and two different transcripts were identified. The promoter P2 is used much more efficiently, and in a stringent, tissue-specific manner. The transcription from P1 takes place in different tissues and stages of development of D. virilis. However, the mRNA transcribed from P1 seems to be inactive in translation as there are three open-reading frames (ORF) between P1 and P2, which may block the translation in P1 initiated mRNA. Insertion of sequence containing the three ORFs into the 5' untranslated region of the CAT gene strongly inhibited expression of CAT. Point mutations destroying the three ORFs completely eliminate the inhibitory effect. Hence tissue-specific expression of the estS gene may depend on control at the level of transcription, promoter selection and translation. |
| Databáze: | OpenAIRE |
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